A0A1S5WJ46 · A0A1S5WJ46_9REOV

  • Protein
    Outer capsid glycoprotein VP7
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Calcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. Following entry into the host cell, low intracellular or intravesicular Ca2+ concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7.
Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors.

Miscellaneous

In group A rotaviruses, VP7 defines the G serotype.
Some rotavirus strains are neuraminidase-sensitive and require sialic acid to attach to the cell surface. Some rotavirus strains are integrin-dependent. Some rotavirus strains depend on ganglioside for their entry into the host cell. Hsp70 also seems to be involved in the entry of some strains.

Features

Showing features for binding site.

132650100150200250300
TypeIDPosition(s)Description
Binding site95Ca2+ 1 (UniProtKB | ChEBI)
Binding site177Ca2+ 2 (UniProtKB | ChEBI)
Binding site206Ca2+ 1 (UniProtKB | ChEBI)
Binding site214Ca2+ 1 (UniProtKB | ChEBI)
Binding site216Ca2+ 1 (UniProtKB | ChEBI)
Binding site228Ca2+ 2 (UniProtKB | ChEBI)
Binding site229Ca2+ 2 (UniProtKB | ChEBI)
Binding site231Ca2+ 2 (UniProtKB | ChEBI)
Binding site301Ca2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum lumen
Cellular Componentmembrane
Cellular ComponentT=13 icosahedral viral capsid
Cellular Componentviral outer capsid
Molecular Functionmetal ion binding

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Outer capsid glycoprotein VP7

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • RVA/Pig-wt/TWN/3-17/2015/G9P23
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Duplornaviricota > Resentoviricetes > Reovirales > Sedoreoviridae > Rotavirus > Rotavirus A

Accessions

  • Primary accession
    A0A1S5WJ46

Subcellular Location

Virion
Note: The outer layer contains 780 copies of VP7, grouped as 260 trimers. Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles.

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane6-23Helical
Transmembrane30-48Helical

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_502323318751-326Outer capsid glycoprotein VP7
Disulfide bond82↔135
Disulfide bond165↔249
Disulfide bond191↔244
Disulfide bond196↔207

Post-translational modification

N-glycosylated.
The N-terminus is blocked possibly by pyroglutamic acid.

Keywords

Interaction

Subunit

Homotrimer. 2 Ca2+ ions bound at each subunit interface in the trimer hold the trimer together.
Homotrimer; disulfide-linked. 2 Ca2+ ions bound at each subunit interface in the trimer hold the trimer together. Interacts with the intermediate capsid protein VP6. Interacts with the outer capsid protein VP5*.

Structure

3D structure databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region165-167CNP motif; interaction with ITGAV/ITGB3
Region237-239LVD motif; interaction with ITGA4/ITGB1 heterodimer
Region253-255GPR motif; interaction with ITGAX/ITGB2

Sequence similarities

Belongs to the rotavirus VP7 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    326
  • Mass (Da)
    37,065
  • Last updated
    2017-05-10 v1
  • Checksum
    0BF6F95D0A52CD11
MYGIEYTTVLTFLISIILLNYILKSLTSAMDFIIYRFLLLIVIVSPLVKSQNYGINLPITGSMDTAYANSSQQETFLTSTLCLYYPTEASTQIGDAEWKNTLSQLFLTKGWPTGSVYFKEYADITSFSIDPQLYCDYNVVLMKHDSTLELDMSELADLILNEWLCNPMDITLYYYQQTDEANKWISMGQSCTIKVCPLNTQTLGIGCTTTDTATFEEVAKNEKLVITDVVDGVNHKLDVTANTCTIRNCKKLGPRENVAIIQVGGSEILDITADPTTAPQTERMMRINWKKWWQVFYTVVDYINQIVQVMSKRSRSLNSAAFYYRV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KU739941
EMBL· GenBank· DDBJ
AQQ12261.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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