A0A1S2N0C5 · A0A1S2N0C5_9MICC
- ProteinPhosphoenolpyruvate-protein phosphotransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids565 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).
Catalytic activity
- L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-L-histidyl-[protein] + pyruvate
Cofactor
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 188 | Tele-phosphohistidine intermediate | ||||
Sequence: H | ||||||
Binding site | 288 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 324 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 413 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 436-437 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: ND | ||||||
Binding site | 437 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 447 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Active site | 490 | Proton donor | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | kinase activity | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoenolpyruvate-protein phosphotransferase activity | |
Biological Process | phosphoenolpyruvate-dependent sugar phosphotransferase system | |
Biological Process | phosphorylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate-protein phosphotransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Micrococcales > Micrococcaceae > Rothia
Accessions
- Primary accessionA0A1S2N0C5
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 5-125 | Phosphotransferase system enzyme I N-terminal | ||||
Sequence: TGVGVSAGRVIGPVLQMPEPIREPDAASTLAEGESPEAAVERLKEAAEAVKENLKERAGRASGNAAAVLKSTALMAGDKALLKNAGKLVTDKGLSPERAIWEAAGDYAAQMKALGGYMAQR | ||||||
Coiled coil | 41-68 | |||||
Sequence: EAAVERLKEAAEAVKENLKERAGRASGN | ||||||
Domain | 154-223 | PEP-utilising enzyme mobile | ||||
Sequence: EFVLVAEDLAPADTATLDPAKVIALVTSEGGPQAHTAILARDLGLPAIVAANGAREIPDGTTVYVDATDG | ||||||
Domain | 255-528 | PEP-utilising enzyme C-terminal | ||||
Sequence: LADGTEIPLYSNVGNGKAAQTAAEAKAEGIGLFRTEFAFLNRDEEPSVEEQTVEYKAVFDAFPGRHIVVRTLDAGADKPLPFLTDTSEPNPALGVRGYRTDWTSPGVLQRQIEAIAAAAKDSTADVWVMAPMISTAPEAHDFSAMLEEAGLKTHGVMVETPSAAVTSRDILHEVDFVSVGTNDLTQYTMAADRLLGPLAELNNPWQPGVLRMIDLTVRGARQAEDETGKAKSVSVCGEAAADPALAVVLVGLGVNALSMNTRSIPAVAAVLKSV |
Sequence similarities
Belongs to the PEP-utilizing enzyme family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length565
- Mass (Da)59,218
- Last updated2017-04-12 v1
- Checksum873F72E4397B9488