A0A1S2MN63 · A0A1S2MN63_9STAP
- ProteintRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
- GenemiaB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids513 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.
Catalytic activity
- N6-dimethylallyladenosine37 in tRNA + [sulfur carrier]-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N6-dimethylallyladenosine37 in tRNA + [sulfur carrier]-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H+
Cofactor
Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 76 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 112 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 146 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 222 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 226 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 229 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | methylthiotransferase activity | |
Biological Process | tRNA methylthiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus
Accessions
- Primary accessionA0A1S2MN63
Proteomes
Subcellular Location
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 67-185 | MTTase N-terminal | ||||
Sequence: KTFLIKTYGCQMNAHDTEVMAGILGALGYTATEDINQADVILINTCAIRENAENKVFSEIGNLKHLKKEKPETVIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHRLPEILEEAYL | ||||||
Domain | 208-438 | Radical SAM core | ||||
Sequence: REGKIKAWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIEEVRDLARQGYQEITLLGQNVNSYGKDIEGLAYGLGDLLEDITQIDIPRVRFTTSHPWDFTDRMIEVIANGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLELVDRIKAKIPNVALTTDIIVGYPNETEAQFEETLSLYEEVEFEHAYTYIYSQRDGTPAAKMKDNVPTDVKKERLQRLNKKVSYYSEQ | ||||||
Domain | 441-504 | TRAM | ||||
Sequence: KRYEGQIVQVLCEGASKKDEHVLAGYTSKNKLVNFKAPNEMIGQLVDVQVDEAKQYSLNGTFLR |
Sequence similarities
Belongs to the methylthiotransferase family. MiaB subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length513
- Mass (Da)58,611
- Last updated2017-04-12 v1
- Checksum322211E844DA741B