A0A1S2MG87 · A0A1S2MG87_9STAP
- ProteinRibosomal RNA small subunit methyltransferase A
- GenersmA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids296 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.
This protein produces a dimethylation of the adenine residue at position 2085 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.
Catalytic activity
- adenosine1518/adenosine1519 in 16S rRNA + 4 S-adenosyl-L-methionine = N6-dimethyladenosine1518/N6-dimethyladenosine1519 in 16S rRNA + 4 S-adenosyl-L-homocysteine + 4 H+
RHEA-COMP:10232 CHEBI:74411 Position: 1518CHEBI:74411 Position: 1519+ 4 CHEBI:59789 = RHEA-COMP:10233 CHEBI:74493 Position: 1518CHEBI:74493 Position: 1519+ 4 CHEBI:57856 + 4 CHEBI:15378
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 30 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 32 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 57 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 78 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 103 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 128 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity | |
Molecular Function | 23S rRNA (adenine(2085)-N(6))-dimethyltransferase activity | |
Molecular Function | RNA binding |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibosomal RNA small subunit methyltransferase A
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus
Accessions
- Primary accessionA0A1S2MG87
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 37-213 | Ribosomal RNA adenine methylase transferase N-terminal | |||
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length296
- Mass (Da)33,478
- Last updated2017-04-12 v1
- ChecksumDDEDEFB0F5B3C224
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
MODY01000008 EMBL· GenBank· DDBJ | OIJ29011.1 EMBL· GenBank· DDBJ | Genomic DNA |