A0A1Q8YZ89 · A0A1Q8YZ89_9ENTR

Function

function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site183-184ATP (UniProtKB | ChEBI)
Binding site265ATP (UniProtKB | ChEBI)
Binding site287ATP (UniProtKB | ChEBI)
Binding site296ATP (UniProtKB | ChEBI)
Active site456Cysteine persulfide intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uracil-4 sulfurtransferase activity
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process
Biological Processthiazole biosynthetic process
Biological ProcesstRNA thio-modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA sulfurtransferase
  • EC number
  • Alternative names
    • Sulfur carrier protein ThiS sulfurtransferase
    • Thiamine biosynthesis protein ThiI
    • tRNA 4-thiouridine synthase

Gene names

    • Name
      thiI
    • ORF names
      CWN49_25440
      , DN619_09560
      , K9I60_18155
      , N5C89_13325
      , N7692_25455
      , NCTC11685_02559
      , NCTC11694_02473
      , NCTC9161_05736
      , OA42_06605

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • CH2
    • A10
    • GEO_33_Up_A
    • NCTC11685
    • NCTC11694
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Klebsiella/Raoultella group > Klebsiella

Accessions

  • Primary accession
    A0A1Q8YZ89

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond344↔456Redox-active

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain61-165THUMP
Domain404-480Rhodanese

Sequence similarities

Belongs to the ThiI family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    482
  • Mass (Da)
    54,701
  • Last updated
    2017-04-12 v1
  • Checksum
    71846CDA206E24AA
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKNYDETLAVVRHWDHIEVRAKDENQRPAIRDALTRIPGIHHILEVEDVPYTSLHDIFEQTLPLWRDALEGKTFCVRVKRRGKHEFTSIEVERYVGGGLNQHIETARVKLTDPDVTVNLEIENDRLLLVKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKVDDGQMGVVLKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISHDKEHIIDLAREIGTEDFARTMPEYCGVISKSPTVKAVKAKIEAEEEHFDFSILEKVVAEASNIDIRDIAQQTEQDVVEVETVSGFGPDDAILDIRSIDEQDDKPLKVEGVDVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFENVKVYRP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JSWW01000013
EMBL· GenBank· DDBJ
KKY75762.1
EMBL· GenBank· DDBJ
Genomic DNA
JAIPKU010000002
EMBL· GenBank· DDBJ
MCZ9452611.1
EMBL· GenBank· DDBJ
Genomic DNA
JAOEEU010000037
EMBL· GenBank· DDBJ
MDH0490950.1
EMBL· GenBank· DDBJ
Genomic DNA
JAOCBF010000016
EMBL· GenBank· DDBJ
MDH0963814.1
EMBL· GenBank· DDBJ
Genomic DNA
PIDR01001031
EMBL· GenBank· DDBJ
PLO64437.1
EMBL· GenBank· DDBJ
Genomic DNA
QKPB01000014
EMBL· GenBank· DDBJ
RWT46077.1
EMBL· GenBank· DDBJ
Genomic DNA
UGJR01000002
EMBL· GenBank· DDBJ
STR41291.1
EMBL· GenBank· DDBJ
Genomic DNA
UGMS01000001
EMBL· GenBank· DDBJ
STV79197.1
EMBL· GenBank· DDBJ
Genomic DNA
UGMW01000003
EMBL· GenBank· DDBJ
STV95792.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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