A0A1P8YVU2 · A0A1P8YVU2_9PAPI

Function

function

ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site486-493ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell nucleus
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionDNA binding
Molecular FunctionDNA helicase activity
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Replication protein E1
  • EC number
  • Alternative names
    • ATP-dependent helicase E1

Gene names

    • Name
      E1

Organism names

  • Taxonomic identifier
  • Strain
    • EhPV3
  • Taxonomic lineage
    Viruses > Monodnaviria > Shotokuvirae > Cossaviricota > Papovaviricetes > Zurhausenvirales > Papillomaviridae > Firstpapillomavirinae > Psipapillomavirus > Psipapillomavirus 3

Accessions

  • Primary accession
    A0A1P8YVU2

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue97Phosphoserine; by host
Modified residue110Phosphoserine; by host

Post-translational modification

Phosphorylated.

Keywords

Expression

Keywords

Interaction

Subunit

Can form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA-binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.

Family & Domains

Features

Showing features for region, compositional bias, motif, domain.

Type
IDPosition(s)Description
Region1-59Disordered
Compositional bias27-55Acidic residues
Motif81-83Nuclear localization signal
Compositional bias133-151Polar residues
Region133-193Disordered
Compositional bias158-188Polar residues
Domain460-610SF3 helicase
Region632-660Disordered

Sequence similarities

Belongs to the papillomaviridae E1 protein family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    660
  • Mass (Da)
    73,756
  • Last updated
    2017-04-12 v1
  • MD5 Checksum
    E5F9CB96FA4A12EE55D81D149F915484
MATSGTDTDPQEGPSGEGFLDREADCSEGGETDEDEGSEASNVSDLIDDDVDEVDNAQGNTLALFQQQQAEDDEKLVSLLKRKHHCTPDTKDLQGLSPRLDAMRISPQRSRVAKKRLFKPGDDSGVELTLTLTNEAADTPASENTLQVEGTLEGRTPGEGQESESTPQSDSLTETPTDPGSEGTGSSQPEPVREGEVLDFATAILRSSNRKACMYARFKELYGIGFNDLVRKFKSDKTCAKAWVIVLYALGGPLYECLKTVLPDHCTYTNMQLQVGRQGGVALMLAEFKTAKSRETVHKLFKSICNASEELMLCEPPNVRSSPAALYWVQKGTTKVADTTGNYPDWIAKQTMISHIMAENTTFDFGVMVQWAYDNNIVEEANIAYQYALYAEEDKNARAWLMTSTQAKHVRDCAVMVRYYKQAEMQDMSMSEWIWSRCKLVSESTGDWKTIVQFLRHQGVEVIPFLRTFRDWLKAIPKRNTICIHGPPNTGKSMFGMSLMKFMSGAVLSYVNSRSQFWLQPIVRAKVAMLDDATGACWTYINVYLRNMLDGNPISVDMKHKAPLQTVCPPLLITSNINISEDENHKYLHSRVKCISFPCPLQLGADGRPTVLITNAHWKSFFEKFTQHLELTPPEEEEDSEHGSSQRAFRCAAREDTEAL

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias27-55Acidic residues
Compositional bias133-151Polar residues
Compositional bias158-188Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KX276956
EMBL· GenBank· DDBJ
AQA28211.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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