A0A1N7EUG7 · A0A1N7EUG7_9GAMM

Function

function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site183-184ATP (UniProtKB | ChEBI)
Binding site265ATP (UniProtKB | ChEBI)
Binding site287ATP (UniProtKB | ChEBI)
Binding site296ATP (UniProtKB | ChEBI)
Active site455Cysteine persulfide intermediate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uracil-4 sulfurtransferase activity
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process
Biological Processthiazole biosynthetic process
Biological ProcesstRNA 4-thiouridine biosynthesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA sulfurtransferase
  • EC number
  • Alternative names
    • Sulfur carrier protein ThiS sulfurtransferase
    • Thiamine biosynthesis protein ThiI
    • tRNA 4-thiouridine synthase

Gene names

    • Name
      thiI
    • ORF names
      SAMN02745664_10783

Organism names

  • Taxonomic identifier
  • Strain
    • DSM 21768
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Moraxellales > Moraxellaceae > Moraxella

Accessions

  • Primary accession
    A0A1N7EUG7

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain61-165THUMP
Domain433-482Rhodanese

Sequence similarities

Belongs to the ThiI family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    484
  • Mass (Da)
    54,486
  • Last updated
    2017-03-15 v1
  • MD5 Checksum
    242887DB25C9CFD6C096F1EEB1FAF0E2
MKFIVKLFPEIIMKSESVRKRFIKVLTGNIKEILQKIDSSVVVIRHWDFIEVRSHNEQYHKALREALQRISGIHHFLEVQESSYQDLHDIFLQTLAEYQDKLINKSFCVRVKRRGKHDFDSMAVMQYVGGGLNQHIKSAKVQLKNPDVTVLLEIDQDKLLLIKERFEGMGGFPMSTQEDVLSLISGGFDSSVSSYMFMRRGSRVHYCFFNLGGKAHEIGVKQMAYHLWSRYSSSHKVKFVTVDFEPVVAEILRSIDDGQMGVVLKRLMLKAASVIAERLGIQALVTGEALGQVASQTLTNLHVIDKAATRLVLRPLITSDKEQIIKLSEQIGTMDIAKSMPEFCGVISKSPTVKAVEQKICAEEEKFDFGILQHAIDTAIISDIRTLAEADGSTLLRPEVVHEVQAGQVVLDIRTPDECDELPLAVDGEVIAVPFYQLSTRFATLDSAKTYLLYCQQGVMSKLQAGRLLEQGHQNVKVLKIIKA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FTNU01000007
EMBL· GenBank· DDBJ
SIR91697.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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