A0A1M5A5Z8 · AGO_MARH1

Function

function

A highly versatile argonaute that uses 5'-phospho- and 5'-OH- guide RNA (gRNA) or DNA (gDNA) to cleave target RNA or ssDNA (tDNA) in all possible combinations; has no detectable activity in the absence of guide. Uses short guide sequences (18-21 nucleotides (nt) on average) to bind complementary target nucleic acids resulting in target cleavage in a site-specific manner. Using 5'-phospho-gRNA or 5'-OH-gRNA the cleavage site is 10 nt downstream of the target residue base-paired with the 5'-end of the gRNA, using 5'-phospho-gDNA the cleavage site is 11 nucleotides (nt) downstream, while with 5'-OH-gDNA the cleavage site is 9 nt downstream.

Miscellaneous

Unlike most prokaryotic argonautes, this is encoded in a CRISPR-cas locus.

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Mn2+ supports cleavage of all guide:target combinations, Mg2+ only supports gRNA-directed cleavage (PubMed:36288702).
Cleavage probaby requires 2 divalent metal cations (By similarity).

Temperature Dependence

Optimum temperature for all combinations of guide:target is 60-65 degrees Celsius, the RNA product is more stable at 60 than 65 degrees Celsius.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site445
Binding site445Mn2+ 1 (UniProtKB | ChEBI)
Binding site445Mn2+ 2 (UniProtKB | ChEBI)
Active site481
Active site515
Binding site515Mn2+ 1 (UniProtKB | ChEBI)
Active site623
Binding site623Mn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA endonuclease activity
Molecular Functionmetal ion binding
Molecular FunctionRNA binding
Molecular FunctionRNA endonuclease activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein argonaute
  • EC number
  • Short names
    MhAgo

Gene names

    • Name
      ago
    • ORF names
      SAMN02745164_02104

Organism names

Accessions

  • Primary accession
    A0A1M5A5Z8

Proteomes

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis445No longer cleaves target; when associated with A-515.
Mutagenesis515No longer cleaves target; when associated with A-445.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004577861-640Protein argonaute

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-100N-terminal domain
Region101-153Linker L1
Region154-209PAZ domain
Region210-291Linker L2
Region292-423Mid domain
Region424-640PIWI domain

Domain

Has 4 domains (N-terminal, PAZ, Mid and PIWI). The N-terminal and PAZ domains are joined by linker L1, the PAZ and Mid domains are joined by linker L2. The domains assemble in 2 lobes; the PAZ lobe consists of the N-terminal, L1, PAZ and L2 domains, while the PIWI lobe has the Mid and PIWI domains. The PIWI domain assumes an RNase H fold and has the catalytic residues. gDNA lies between the 2 lobes, with its unpaired 5'-end anchored in the Mid lobe.

Sequence similarities

Belongs to the argonaute family. Long pAgo subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    640
  • Mass (Da)
    76,715
  • Last updated
    2017-03-15 v1
  • Checksum
    E1527C6F01E12279
MYLNLYEIKIPYRVKRLYYFNKENDPKEFARNLSRVNNIRFNDSKDLVWLEIPDIDFKITPQQAEKYKIEKNEIIGEKEDSDLFVKTIYRYIKKKFIDNNFYYKRGNNYISINDKFPLDSNTNVNAHLTYKIKLYKINERYYISVLPKFTFLSDKPALESPIKSTYLFNIKSGKTFPYISGLNGVLKIDLGENGIKEVLFPENYYFNFTSKEAEKFGFSKEIHNIYKEKIFSGYKKIKQSLYFLEDIININNYNLTMDKKIYVNIEYEFKKGISRNIKDVFKYSFYKNDQKIKIAFFFSSKKQIYEIQRSLKMLFQNKNSIFYQTIYEMGFSKVIFLREPKTNSSAFMYNPETFEISNKDFFENLEGNIMAIIILDKFLGNIDSLIQKFPENLILQPILKEKLEKIQPYIIKSYVYKMGNFIPECQPYVIRNLKDKNKTLYIGIDLSHDNYLKKSNLAISAVNNFGDIIYLNKYKNLELNEKMNLDIVEKEYIQILNEYYERNKNYPENIIVLRDGRYLEDIEIIKNILNIENIKYSLIEVNKSVNINSCEDLKEWIIKLSDNNFIYYPKTYFNQKGVEIKIIENNTDYNNEKILEQVYSLTRVVHPTPYVNYRLPYPLQVVNKVALTELEWKLYIPYMK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FQUI01000053
EMBL· GenBank· DDBJ
SHF25574.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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