A0A1L9WUI4 · ANEE_ASPA1

Function

function

Thiohydrolase; part of the gene cluster that mediates the biosynthesis of aculenes, a unique type of norsesquiterpenes that contain a nordaucane skeleton linked to an L-proline moiety and are of mixed biosynthetic origin (PubMed:31618514).
The pathway begins with the synthesis of dauca-4,7-diene by the terpene cyclase aneC using farnesyl pyrophosphate (FPP) as substrate (PubMed:31618514).
The cytochrome P450 monooxygenase aneF then performs the initial oxidation at C-12 of dauca-4,7-diene to yield asperaculane D (PubMed:31618514).
Asperaculane D is substrate of the cytochrome P450 monooxygenase aneD for C-10 hydroxylation to yield asperaculane E (PubMed:31618514).
The cytochrome P450 monooxygenase aneG then converts asperaculane E into aculene D via C-2 oxidation (PubMed:31618514).
The monomodular nonribosomal peptide synthtase aneB adenylates L-proline and the thiohydrolase aneE transfers this activated L-proline derivative to aculenes D and C to produce respectively aculenes B and A (PubMed:31618514).
The dioxygenase aneA converts aculene D into aculene C, and aculene B into aculene A by introducing the 5,6-alkene moiety (PubMed:31618514).
Asperculanes A, B, C and F, as well as 14-prolyl asperculane C, might be shunt products of the pathway (PubMed:31618514).

Catalytic activity

Pathway

Secondary metabolite biosynthesis.

GO annotations

AspectTerm
Molecular Functionhydrolase activity

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Thiohydrolase aneE
  • EC number
  • Alternative names
    • Aculenes biosynthesis cluster protein E

Gene names

    • Name
      aneE
    • ORF names
      ASPACDRAFT_78840

Organism names

Accessions

  • Primary accession
    A0A1L9WUI4

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Abolishes the production of aculenes A and B and accumulates aculenes C and D.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004490961-318Thiohydrolase aneE

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    318
  • Mass (Da)
    35,671
  • Last updated
    2017-03-15 v1
  • Checksum
    3F55884F7927A802
MTKDNEEKTYSEVEFLTVDGLKLRGRLYLGEANGPALVMAHGFNAVKEIVIPWAAEVFQKNGISVLLFDPRNYGESEGMPRQEVDPEKQIEDYFDAVTFLRQQPGIDPEAIGLWGVSTSGATAIGAACFDKRVRLIISVCPLIEATFREEMVPDVMAQIIREREALVAARTAGQQSPQPTLVPMINQVGVSPMGFNAIHGKNFYEEMPWFKETAPNFRPHTTTLTYYKMLRWHPLSNIRCLSPTPIQMLIPGKDDVCPTQEQLDFFEALPGPKRMEYYEDRNHHGLLLGSAFEGVMEAQVRFVQDVLAGKFALKSGSQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KV878977
EMBL· GenBank· DDBJ
OJJ99916.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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