A0A1L9WQQ1 · ACRE_ASPA1

Function

function

FAD-binding monooxygenase; part of the cluster that mediates the biosynthesis of acurin A, a highly reduced polyketide coupled to a serine via a peptide bond (PubMed:32234543).
The activities of the highly reducing polyketide synthase acrA and the nonribosomal peptide synthetase acrB are collectively responsible for the synthesis of the acurin A core structure with a heptaketide backbone produced by acrA covalently fused to a L-serine by acrB (PubMed:32234543).
After the formation of the PK-NRP hybrid product, it is detached from acrB by reductive release to set up the formation of the lactam ring by aldol condensation (Probable). The hydrolyase acrC then catalyzes water loss to generate a double bond in the ring (Probable). This double bond is probably reduced, which is followed by three oxidations at C-22 to generate the carboxylic acid moiety, involving probably the FAD-binding monooxygenase acrE and the cytochrome P450 monooxygenases acrD and acrF (Probable). Finally, a last methylation step performed by the O-methyltransferase acrG leads to the production of acurin A (Probable)

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 1 FAD per subunit.

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site61-64FAD (UniProtKB | ChEBI)
Binding site71-73NADP+ (UniProtKB | ChEBI)
Binding site73-74FAD (UniProtKB | ChEBI)
Binding site79FAD (UniProtKB | ChEBI)
Binding site200-206NADP+ (UniProtKB | ChEBI)
Binding site223-224NADP+ (UniProtKB | ChEBI)
Site346Transition state stabilizer

GO annotations

AspectTerm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionN,N-dimethylaniline monooxygenase activity
Molecular FunctionNADP binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    FAD-binding monooxygenase acrE
  • EC number
  • Alternative names
    • Acurin A biosynthesis cluster protein E

Gene names

    • Name
      acrE
    • ORF names
      ASPACDRAFT_122285

Organism names

Accessions

  • Primary accession
    A0A1L9WQQ1

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Abolishes the production of acurin A.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004504251-593FAD-binding monooxygenase acrE

Expression

Induction

Expression is positively regulated by the acurin A cluster-specific transcription regulator acrR.

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the FAD-binding monooxygenase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    593
  • Mass (Da)
    66,932
  • Last updated
    2017-03-15 v1
  • Checksum
    E660C64861D292B4
MHRELKEFSHPHGSTGIYEDNLQVDVLIVGAGFSGIYMLHELRKQGLKTVVYEAGSDLGGTWRFNCYPGARVDSQVPLYQFSIPETYKDWTWSTNYPDYKELRAYFDHVDKVLDIKKDVAFGSVVVDAQFDTSQSRWVVKTQDGRTAYAKYFIAAAGVSSKRYIPEWEGIENFKGPIHHTSFWPEHEVDVRGKRAAIIGTGASGVQVTQAWGPQAGHLKVFLRSPNYSIPMRRKELTADEQNQLKPIYPQLFDLREKTFTGALVDFSERSALEDSEAEREAFYEQRYQTGGFDFSTANYKDTMLNPVANRYLYDFWAKKTRARLNDERVKDLLAPVEPPYFFGGKRSSLETDFYEQFNRDTVELVDAKSNPIVGFTENGIKMQDGTVHEVDVVCLATGFDTTTGSMINLGVRSIHGTTLQEDWSQAAETYLGLTVSGYPNLFHLYGTHAPTLFSQAVTTIEVQGRWIVDAIKQMERQAIRSINPSREAAHAWKLQIRAFQNASFFPTVHSTYMGGSIPGKPFELVSYPAGMPMYARQIRDALPTFPGFEVVKADGEKVENLAPGGLEATTNFFERLFGMPPSSAKEDLAKQDH

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KV878980
EMBL· GenBank· DDBJ
OJJ98492.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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