A0A1L9WQK2 · VRCB_ASPA1

Function

function

Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of the sesterterpene variecolin (PubMed:38261827).
The first step in the pathway is performed by the variecoladiene synthase vrcA that possesses both prenyl transferase and terpene cyclase activity, converting isopentenyl diphosphate and dimethylallyl diphosphate into geranylfarnesyl pyrophosphate (GFPP) and then converting GFPP into the tetracyclic variecoladiene (PubMed:38261827).
The cytochrome P450 monooxygenase vrcB then catalyzes multiple oxidations at C-5 and C-20 positions to yield variecolin (PubMed:38261827).

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Biotechnology

Variecolin and its derivatives produced by employing P450 monooxygenases from other fungal terpenoid pathways exhibit anticancer activity, thus, the skeleton of variecolin could be considered a privileged scaffold for developing anticancer agents.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site470Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome P450 monooxygenase vrcB
  • EC number
  • Alternative names
    • Variecolin biosynthesis cluster protein B

Gene names

    • Name
      vrcB
    • ORF names
      ASPACDRAFT_53318

Organism names

Accessions

  • Primary accession
    A0A1L9WQK2

Proteomes

Organism-specific databases

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane5-27Helical

Keywords

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004605511-528Cytochrome P450 monooxygenase vrcB

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    528
  • Mass (Da)
    60,829
  • Last updated
    2017-03-15 v1
  • Checksum
    DD7C9E4DA12D9D1E
MYGIYGLFFAAVALYSVALVIYRLYLHPLSRFPGPKIAAATGWYEFYHEVIRGGMYIHEIQRMHREYGPIIRINPYEIVINDPDYYTTVYVASNTRRSEKWYTLQGTGLEGKALSFFSHFRTCRTRMHMTDQIVGAMGMTMGHELHRRRRKHFDPFFSRLGVTQIEGVILDEIQLLTDRLEGNRKSGRTIQMEHVMAAFTGDIVTKICSEKSPDMIRHPDFGKEWYVTIHTYQLQVHLFVQFPYLISLTQLIPRGLVMHFSPGAAAFKSLHQYAFDHITEAKKDMGRAVKVQQEAGRSSVFRHVLSSDMPGTERETERLAREALQLFGAGTATLVRAFSMIFYHVLSDPQMRGQLREELKDIMAGYPAKRPTWQELERLPYLHGIVKEGLRLSYGVMRHLARVSPDQPLQFREWTIPAGTPVGMSSHSLHSDPETFPEPARFLPERWMKDRHHPNMNRNWVPFARGSRMCIGMNLALAEMYWVLAVLFRPGAPQLELFETTEADVVPVRDYVGGIPEFGTKGVRVRVV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KV878980
EMBL· GenBank· DDBJ
OJJ98432.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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