A0A1L8GLK3 · RFW3L_XENLA

Function

function

E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (By similarity).
Plays a key role in RPA-mediated DNA damage signaling and repair (By similarity).
Required to translesion DNA synthesis across DNA-protein cross-link adducts by catalyzing ubiquitination of proteins on single-stranded DNA (ssDNA) (PubMed:33321094).
Mediates ubiquitination of the hmces DNA-protein cross-link, possibly promoting its degradation (PubMed:33321094).

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster.

Pathway

Protein modification; protein ubiquitination.

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular ComponentPML body
Molecular Functionmetal ion binding
Molecular Functionubiquitin protein ligase activity
Biological Processinterstrand cross-link repair
Biological Processprotein ubiquitination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    E3 ubiquitin-protein ligase rfwd3.L
  • EC number
  • Alternative names
    • RING finger and WD repeat domain-containing protein 3.L

Gene names

    • Name
      rfwd3.L

Organism names

  • Taxonomic identifier
  • Strain
    • J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus

Accessions

  • Primary accession
    A0A1L8GLK3

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Nucleus, PML body
Cytoplasm
Note: In undamaged cells, found both in the cytoplasm and in the nucleus, partially associated with PML nuclear bodies. In response to replication block, such as that caused by hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to stalled replication forks or to sites of DNA repair.

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004597431-751E3 ubiquitin-protein ligase rfwd3.L

Proteomic databases

Expression

Gene expression databases

Family & Domains

Features

Showing features for region, compositional bias, zinc finger, repeat.

TypeIDPosition(s)Description
Region1-33Disordered
Compositional bias17-31Polar residues
Region97-188Disordered
Compositional bias115-142Polar residues
Compositional bias170-186Acidic residues
Zinc finger264-308RING-type; degenerate
Repeat471-511WD 1
Repeat513-553WD 2
Repeat559-604WD 3

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    751
  • Mass (Da)
    82,410
  • Last updated
    2017-03-15 v1
  • Checksum
    934CFDA861504586
MAQEEMEVDLPNLLGPMDSNRVSTSHQPLGSRQVLFLPPPITYSDDSEELFLGPTEPHDVIVLDSGEEAPAQVVAPTNPLQMLHGALEQLRQAAELRSSVRRTRVQRRSTRRQQRGGSQRTAGTSSRAALSSFFQINRTQGLAPTLEREERNTVLRTESLRTLPGDSSDETVELSEEEEGEGSSTDVDEVEINAGAAPPAEPAPEELQINVVEVQAEASAVSDSLPIPERVTLPLQIQTKQKTPVKLLSPVKPLPPEEDEGDTCAICFEPWTNAGQHRLSALRCGHLFGFTCIERWLKGGASKCPQCNKKAKRADIVVLYARSLKALDTSEQERMKSSLEMEQSMRRKAQLESAQCRLQVQVLTDECGKLRNQIQELKMLIAQHGTGTSVQASSSRSTISGSLSSSQGQHKYSFEKAILISQTGSCRVMSYCESMSCLVVSQPSPQTTLIPGCGIKKLSAANLKSSQYVPIHSKQIRGLAFSNRSEGLLLSAALDNTVKLTSLLTNTVVQTYNTGRPVWSCCWCSDNSNYVYAGLINGSVLVYDLRDTSNCVQELVPLGSRCPVVSLSYVPRAASEVFPCGGVLVGTLEGACFWEMKDDQYRPHVLPLEPGGCTDIQIESNTRHCLVTYRPGKNYNFVRGVMMELTSNRLNDSEDEYSCSCYPVQTFNAGSTCKLLTKNAIFQSPERDGTVLVCAGDEASNSAMLWHSGNGTLLQKLQADQPVLDICPMEVNQSSMLATLTEKMVKIYKWE

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias17-31Polar residues
Compositional bias115-142Polar residues
Compositional bias170-186Acidic residues

Keywords

Sequence databases

Genome annotation databases

Similar Proteins

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