A0A1L8FZ98 · PEX2_XENLA

Function

function

E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling (PubMed:35931083).
The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX2 also regulates peroxisome organization by acting as a E3 ubiquitin-protein ligase (By similarity).

Catalytic activity

  • [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine.
    EC:2.3.2.36 (UniProtKB | ENZYME | Rhea)
  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site245Zn2+ 1 (UniProtKB | ChEBI)
Binding site248Zn2+ 1 (UniProtKB | ChEBI)
Binding site260Zn2+ 2 (UniProtKB | ChEBI)
Binding site262Zn2+ 2 (UniProtKB | ChEBI)
Binding site265Zn2+ 1 (UniProtKB | ChEBI)
Binding site268Zn2+ 1 (UniProtKB | ChEBI)
Binding site281Zn2+ 2 (UniProtKB | ChEBI)
Binding site284Zn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentperoxisomal membrane
Molecular Functionubiquitin protein ligase activity
Molecular Functionzinc ion binding
Biological Processpexophagy
Biological Processprotein import into peroxisome matrix, receptor recycling
Biological Processprotein ubiquitination
Biological Processresponse to amino acid starvation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Peroxisome biogenesis factor 2
  • EC number
  • Alternative names
    • Peroxin-2

Gene names

    • Name
      pex2.L

Organism names

  • Taxonomic identifier
  • Strain
    • J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus

Accessions

  • Primary accession
    A0A1L8FZ98

Proteomes

Organism-specific databases

Subcellular Location

Peroxisome membrane
; Multi-pass membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-15Peroxisomal matrix
Transmembrane16-42Helical; Name=TM1
Topological domain43-48Cytoplasmic
Transmembrane49-74Helical; Name=TM2
Topological domain75-98Peroxisomal matrix
Transmembrane99-125Helical; Name=TM3
Topological domain126-134Cytoplasmic
Transmembrane135-161Helical; Name=TM4
Topological domain162-188Peroxisomal matrix
Transmembrane189-212Helical; Name=TM5
Topological domain213-306Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004569841-306Peroxisome biogenesis factor 2

Proteomic databases

Expression

Gene expression databases

    • 108719303Expressed in muscle tissue and 19 other cell types or tissues

Interaction

Subunit

Component of the PEX2-PEX10-PEX12 retrotranslocation channel.

Protein-protein interaction databases

Family & Domains

Features

Showing features for zinc finger.

TypeIDPosition(s)Description
Zinc finger245-285RING-type

Domain

The three subunits of the retrotranslocation channel (PEX2, PEX10 and PEX12) coassemble in the membrane into a channel with an open 10 Angstrom pore. The RING-type zinc-fingers that catalyze PEX5 receptor ubiquitination are positioned above the pore on the cytosolic side of the complex.

Sequence similarities

Belongs to the pex2/pex10/pex12 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    306
  • Mass (Da)
    35,374
  • Last updated
    2017-03-15 v1
  • Checksum
    E20B87750FF05CB0
MAASENNMEEINPVLRISQLDAIELNKALEQLIWSQFSSCFQGFKPGLLTRFEPEIKASLCLFLWRYTIYTKNATVGQTILNMQYKNDLAVTKKYRPLNKQQKVWFALFLVGGKWLEERSFDLFSNHPFGASFQRTKYFLNAISGLLKFGALLNFLIFLQQGKFATLTERLLGIRSVFSRPQDVRQVGFEYMNREILWHGFAEFLIFLLPLINTQKLKSKLFSWCKPAKSHGVSDPSLAVICKECCLCGEWPAMPHTIGCSHVFCYYCIKSNYMSDMYFTCPKCSSQVHNLQPLEFKIEISEVHTL

Keywords

Sequence databases

Genome annotation databases

Similar Proteins

Disclaimer

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