A0A1L7VZE7 · GUDIS_FUSPR
- Protein(1R,4R,5S)-(-)-guaia-6,10(14)-diene synthase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids401 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the conversion of (2E,6E)-farnesyl diphosphate (FPP) to yield the bicyclic sesquiterpene guaia-6,1014-diene via a 1,10-cyclization, which requires the abstraction of the pyrophosphate from FPP to yield the (E,E)-germacradienyl cation. The only accepted substrate is farnesyl diphosphate (FPP).
Catalytic activity
- (2E,6E)-farnesyl diphosphate = (1R,4R,5S)---guaia-6,1014-diene + diphosphate
Cofactor
Note: Binds 3 Mg2+ ions per subunit.
Pathway
Secondary metabolite biosynthesis; terpenoid biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 134 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 139 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 139 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 242 | substrate | |||
Binding site | 288 | Mg2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 292 | Mg2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 295 | substrate | |||
Binding site | 296 | Mg2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 375-376 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | lyase activity | |
Molecular Function | metal ion binding | |
Biological Process | terpenoid biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name(1R,4R,5S)-(-)-guaia-6,10(14)-diene synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Nectriaceae > Fusarium > Fusarium fujikuroi species complex
Accessions
- Primary accessionA0A1L7VZE7
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000443319 | 1-401 | (1R,4R,5S)---guaia-6,1014-diene synthase | ||
Structure
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-21 | Disordered | |||
Motif | 134-138 | DDXXD motif | |||
Domain
The Asp-Asp-Xaa-Xaa-Asp (DDXXD) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similarities
Belongs to the terpene synthase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length401
- Mass (Da)45,857
- Last updated2017-03-15 v1
- ChecksumF454D54E9DDBBB5B
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FJOF01000009 EMBL· GenBank· DDBJ | CZR45361.1 EMBL· GenBank· DDBJ | Genomic DNA |