A0A1L6Z3A0 · TPS8_PSEAD
- ProteinPseudolaratriene synthase, chloroplastic
- GeneTPS8
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids846 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Converts geranylgeranyl diphosphate to an new 5,7-fused bicyclic diterpene, named pseudolaratriene (PubMed:28096378).
Catalyzes the first committed step in pseudolaric acid B (PAB) biosynthesis (PubMed:28096378).
PAB exhibits antiproliferative activity by inhibiting microtubule polymerization, and has demonstrated antitumor properties against several cancer types (Probable)
Catalyzes the first committed step in pseudolaric acid B (PAB) biosynthesis (PubMed:28096378).
PAB exhibits antiproliferative activity by inhibiting microtubule polymerization, and has demonstrated antitumor properties against several cancer types (Probable)
Catalytic activity
- (2E,6E,10E)-geranylgeranyl diphosphate = diphosphate + pseudolaratrieneThis reaction proceeds in the forward direction.
Cofactor
Pathway
Terpene metabolism.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | magnesium ion binding | |
Molecular Function | terpene synthase activity | |
Biological Process | diterpenoid biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePseudolaratriene synthase, chloroplastic
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Pinopsida > Pinidae > Conifers I > Pinales > Pinaceae > Pseudolarix
Accessions
- Primary accessionA0A1L6Z3A0
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 558 | Abolishes catalytic activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 564 | No effect on catalytic activity. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 564 | Slightly reduces catalytic activity. | ||||
Sequence: Y → H | ||||||
Mutagenesis | 564 | Abolishes catalytic activity. | ||||
Sequence: Y → I, T, or V | ||||||
Mutagenesis | 564 | Almost abolishes catalytic activity. | ||||
Sequence: Y → W | ||||||
Mutagenesis | 584 | Slightly reduces catalytic activity. | ||||
Sequence: F → Y | ||||||
Mutagenesis | 591 | Abolishes catalytic activity. | ||||
Sequence: A → Q | ||||||
Mutagenesis | 670 | Slightly reduces catalytic activity. | ||||
Sequence: H → A, W, or Y | ||||||
Mutagenesis | 696 | Slightly reduces catalytic activity. | ||||
Sequence: S → I or V | ||||||
Mutagenesis | 697 | Strongly reduces catalytic activity. | ||||
Sequence: G → S | ||||||
Mutagenesis | 701 | Reduces catalytic activity 2-fold. | ||||
Sequence: A → C | ||||||
Mutagenesis | 701 | Abolishes catalytic activity. | ||||
Sequence: A → L | ||||||
Mutagenesis | 735 | Abolishes catalytic activity. | ||||
Sequence: G → D, N, or W | ||||||
Mutagenesis | 735 | Slightly reduces catalytic activity. | ||||
Sequence: G → S | ||||||
Mutagenesis | 743 | Strongly reduces catalytic activity. | ||||
Sequence: A → T |
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-58 | Chloroplast | ||||
Sequence: MSRFTSATHGLNLSIKMPISVSQVPSIRSNTSKYELQKLRSTGRSVLQTRRQLAIINM | ||||||
Chain | PRO_0000450341 | 59-846 | Pseudolaratriene synthase, chloroplastic | |||
Sequence: TKRSEADDNDGVERRKGVFHPNLWDDGFIQSLSTVYHEQASYRERAERLIGEVKAVFDSISMGDGDQFISPSAYDTAWVARVPAIDGSSRPQFPQAIDWILLNQQQDGSWGSQSHLSLTHRLTDTLACVIALASWKIESVQIDEGLDFITRGVEKLQSESVPAEFEIIFAELLNQAKSLQLSLPYEHSCLQSLWRKQEPILANGLMDSVAKRSLSSLEEMQDHRMNTDSDGTMHVESFLSSPAVAARVLMRTGNPICLAYLNNVLNKFGDYVPGMYPVDLFQRLWMVDNVERLGIDRHFKKEIQVTLDYVYSYWNGKGIGCGRDSLSPDLNSTSLGFRTLRLHGYNVSADVLEHFKDRDGKFVCSSNPTVGEIRSVLNLYRASLLAFPGEKVMEEAETFARRYLEEIVQKIPPSKFSREIEYVLEFGWQSTVPRWEARSYIDFHGLDTYSPWTIYEMASEKFLELAKLEFNIFNSLQHTELQYLSRWWNDSGMSQMRFTRHRNVEYYTMASCIAMEPSQSAFRIGFTKLCGIATCIDDIYDTYGTIDELKLFREAVKRWDPSAIESLPEYMKSVYMVLYELVNEMAQDTERTQGRDTLDYARNAWEAIIDAHLVEAEWIASGHIPTFEEYLENSKVTSGLHIAILPILTLDVPLPDQLPLQEIDTLSRFHHLASTIGRLSGDMNAYKIDLAHGEESSCISCYMKDNPGTTEGDAHNYANVTISYLMKELNLELMGQHNRVSFLRTSKKPAFDIYRASNYMYKYRDGYTIADKETKNLVMRTLVQAVSL |
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length846
- Mass (Da)96,588
- Last updated2017-03-15 v1
- Checksum3F010A445914E071
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KU685114 EMBL· GenBank· DDBJ | APT40486.1 EMBL· GenBank· DDBJ | mRNA |