A0A1L6Z3A0 · TPS8_PSEAD

Function

function

Converts geranylgeranyl diphosphate to an new 5,7-fused bicyclic diterpene, named pseudolaratriene (PubMed:28096378).
Catalyzes the first committed step in pseudolaric acid B (PAB) biosynthesis (PubMed:28096378).
PAB exhibits antiproliferative activity by inhibiting microtubule polymerization, and has demonstrated antitumor properties against several cancer types (Probable)

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Terpene metabolism.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site595Mg2+ 1 (UniProtKB | ChEBI)
Binding site595Mg2+ 2 (UniProtKB | ChEBI)
Binding site599Mg2+ 1 (UniProtKB | ChEBI)
Binding site599Mg2+ 2 (UniProtKB | ChEBI)
Binding site747Mg2+ 3 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Molecular Functionmagnesium ion binding
Molecular Functionterpene synthase activity
Biological Processditerpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pseudolaratriene synthase, chloroplastic
  • EC number
  • Alternative names
    • Terpene synthase 8
      (PxaTPS8
      )

Gene names

    • Name
      TPS8

Organism names

Accessions

  • Primary accession
    A0A1L6Z3A0

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis558Abolishes catalytic activity.
Mutagenesis564No effect on catalytic activity.
Mutagenesis564Slightly reduces catalytic activity.
Mutagenesis564Abolishes catalytic activity.
Mutagenesis564Almost abolishes catalytic activity.
Mutagenesis584Slightly reduces catalytic activity.
Mutagenesis591Abolishes catalytic activity.
Mutagenesis670Slightly reduces catalytic activity.
Mutagenesis696Slightly reduces catalytic activity.
Mutagenesis697Strongly reduces catalytic activity.
Mutagenesis701Reduces catalytic activity 2-fold.
Mutagenesis701Abolishes catalytic activity.
Mutagenesis735Abolishes catalytic activity.
Mutagenesis735Slightly reduces catalytic activity.
Mutagenesis743Strongly reduces catalytic activity.

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-58Chloroplast
ChainPRO_000045034159-846Pseudolaratriene synthase, chloroplastic

Expression

Tissue specificity

Expressed in young and mature roots (PubMed:28096378).
Expressed at low levels in barks (PubMed:28096378).

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif595-599DDXXD motif

Sequence similarities

Belongs to the terpene synthase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    846
  • Mass (Da)
    96,588
  • Last updated
    2017-03-15 v1
  • Checksum
    3F010A445914E071
MSRFTSATHGLNLSIKMPISVSQVPSIRSNTSKYELQKLRSTGRSVLQTRRQLAIINMTKRSEADDNDGVERRKGVFHPNLWDDGFIQSLSTVYHEQASYRERAERLIGEVKAVFDSISMGDGDQFISPSAYDTAWVARVPAIDGSSRPQFPQAIDWILLNQQQDGSWGSQSHLSLTHRLTDTLACVIALASWKIESVQIDEGLDFITRGVEKLQSESVPAEFEIIFAELLNQAKSLQLSLPYEHSCLQSLWRKQEPILANGLMDSVAKRSLSSLEEMQDHRMNTDSDGTMHVESFLSSPAVAARVLMRTGNPICLAYLNNVLNKFGDYVPGMYPVDLFQRLWMVDNVERLGIDRHFKKEIQVTLDYVYSYWNGKGIGCGRDSLSPDLNSTSLGFRTLRLHGYNVSADVLEHFKDRDGKFVCSSNPTVGEIRSVLNLYRASLLAFPGEKVMEEAETFARRYLEEIVQKIPPSKFSREIEYVLEFGWQSTVPRWEARSYIDFHGLDTYSPWTIYEMASEKFLELAKLEFNIFNSLQHTELQYLSRWWNDSGMSQMRFTRHRNVEYYTMASCIAMEPSQSAFRIGFTKLCGIATCIDDIYDTYGTIDELKLFREAVKRWDPSAIESLPEYMKSVYMVLYELVNEMAQDTERTQGRDTLDYARNAWEAIIDAHLVEAEWIASGHIPTFEEYLENSKVTSGLHIAILPILTLDVPLPDQLPLQEIDTLSRFHHLASTIGRLSGDMNAYKIDLAHGEESSCISCYMKDNPGTTEGDAHNYANVTISYLMKELNLELMGQHNRVSFLRTSKKPAFDIYRASNYMYKYRDGYTIADKETKNLVMRTLVQAVSL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KU685114
EMBL· GenBank· DDBJ
APT40486.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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