A0A1J7IAI2 · A0A1J7IAI2_9PEZI

Function

function

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm5 tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.

Pathway

tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.

Features

Showing features for binding site.

140350100150200250300350400
TypeIDPosition(s)Description
Binding site57-59ATP (UniProtKB | ChEBI)
Binding site63ATP (UniProtKB | ChEBI)
Binding site89ATP (UniProtKB | ChEBI)
Binding site167ATP (UniProtKB | ChEBI)
Binding site172ATP (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionhydrolase activity
Molecular Functionnucleotidyltransferase activity
Molecular FunctiontRNA binding
Biological Processprotein urmylation
Biological ProcesstRNA thio-modification
Biological ProcesstRNA wobble uridine modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytoplasmic tRNA 2-thiolation protein 1
  • EC number
  • Alternative names
    • Cytoplasmic tRNA adenylyltransferase 1

Gene names

    • Name
      NCS6
    • Synonyms
      CTU1
    • ORF names
      CONLIGDRAFT_636641

Organism names

  • Taxonomic identifier
  • Strain
    • NRRL 30616
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Sordariomycetidae > Coniochaetales > Coniochaetaceae > Coniochaeta

Accessions

  • Primary accession
    A0A1J7IAI2

Proteomes

Subcellular Location

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain53-233tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal
Domain371-402Cytoplasmic tRNA 2-thiolation protein 1 C-terminal

Sequence similarities

Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    403
  • Mass (Da)
    44,003
  • Last updated
    2017-02-15 v1
  • Checksum
    A70590BA2FE3611F
MAPTLCAACKTQRALIKRPKNHHRLCKSCFISVFEDEVHHTITSSKLFFPGEKVAIGASGGKDSTVLASVLKTLNERHKYGLDLVLLSIDEGIKGYRDDSLETVKRNATQYDMPLKIVGYDELYGWTMDQVVETIGKKGNCTYCGVFRRQALDRGAKMLGIKHVVTGHNADDVAETVMMNLLRGDLARLSRSTSIVTGDSNSDVKRSKPLKYAYEKEIVLYAHHKKLDYFSTECIYSPEAFRGSARGLIKNLERVRPTAILDIVRSGEDMARLVPVNTTKSCACNGEAAPVVVVAEEDGVGGCGSANGRSSGGEMADMEKQLRQNEAAQGLEVDISAEQPPRIPKSKANAVTLPIRSNGIAAKVLGKQTLGTCERCGYMSSQAICQACMLLEGLNKNRPQIQI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KV875103
EMBL· GenBank· DDBJ
OIW24461.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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