A0A1J4SFH4 · A0A1J4SFH4_9BACT
- ProteinNitrogenase iron protein
- GenenifH
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids276 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.
Catalytic activity
- N2 + 8 reduced [2Fe-2S]-[ferredoxin] + 16 ATP + 16 H2O = H2 + 8 oxidized [2Fe-2S]-[ferredoxin] + 2 NH4+ + 16 ADP + 16 phosphate + 6 H+
CHEBI:17997 + 8 RHEA-COMP:10001 + 16 CHEBI:30616 + 16 CHEBI:15377 = CHEBI:18276 + 8 RHEA-COMP:10000 + 2 CHEBI:28938 + 16 CHEBI:456216 + 16 CHEBI:43474 + 6 CHEBI:15378
Cofactor
Note: Binds 1 [4Fe-4S] cluster per dimer.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 8-15 | ATP (UniProtKB | ChEBI) | |||
Binding site | 94 | [4Fe-4S] cluster (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 131 | [4Fe-4S] cluster (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | nitrogenase activity | |
Biological Process | nitrogen fixation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNitrogenase iron protein
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Thermodesulfobacteriota > Desulfovibrionia > Desulfovibrionales > Desulfovibrionaceae
Accessions
- Primary accessionA0A1J4SFH4
Proteomes
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 97 | ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase | |||
Post-translational modification
The reversible ADP-ribosylation of Arg inactivates the nitrogenase reductase and regulates nitrogenase activity.
The reversible ADP-ribosylation of Arg-97 inactivates the nitrogenase reductase and regulates nitrogenase activity.
Keywords
- PTM
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length276
- Mass (Da)30,056
- Last updated2017-02-15 v1
- MD5 ChecksumB7BFCF55D919EB0AC700F9578473D2D2
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
MNUQ01000182 EMBL· GenBank· DDBJ | OIN98219.1 EMBL· GenBank· DDBJ | Genomic DNA |