A0A1J4NFE0 · A0A1J4NFE0_9GAMM

Function

function

Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

pyridoxal 5'-phosphate (UniProtKB | Rhea| CHEBI:597326 )

Pathway

Cofactor biosynthesis; iron-sulfur cluster biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site75-76pyridoxal 5'-phosphate (UniProtKB | ChEBI)
Binding site156pyridoxal 5'-phosphate (UniProtKB | ChEBI)
Binding site184pyridoxal 5'-phosphate (UniProtKB | ChEBI)
Binding site204-206pyridoxal 5'-phosphate (UniProtKB | ChEBI)
Binding site244pyridoxal 5'-phosphate (UniProtKB | ChEBI)
Active site329Cysteine persulfide intermediate
Binding site329[2Fe-2S] cluster (UniProtKB | ChEBI); ligand shared with IscU; via persulfide group

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Functioncysteine desulfurase activity
Molecular Functionmetal ion binding
Molecular Functionpyridoxal phosphate binding
Biological Process[2Fe-2S] cluster assembly

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cysteine desulfurase IscS
  • EC number

Gene names

    • Name
      iscS
    • ORF names
      BK820_07320

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • LCT-H3
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Moraxellales > Moraxellaceae > Acinetobacter

Accessions

  • Primary accession
    A0A1J4NFE0

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue207N6-(pyridoxal phosphate)lysine

Interaction

Subunit

Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain5-369Aminotransferase class V

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    405
  • Mass (Da)
    44,884
  • Last updated
    2017-02-15 v1
  • Checksum
    E07885276E898C4A
MKRPIYLDYAATTPVDPQVAERMMECLTFEGTFGNAASRSHAYGWQAEEKVEYAREQVANLIKADPREIVWTSGATESDNLALKGVAQFYASKGKHIITSKIEHKAILDTCRELESEGFEITYLEPQPKTGLITPDMVEAAIRPDTILVSLMMVNNEIGTVTDVAAIGEITRAKKIFFHVDAAQAAGKVEIDLSQLKVDLMSFSAHKIYGPKGIGALFVRRSPRVRLKAQMHGGGHERGMRSGTLATHQIVGMGEAFELAGKNLHSEQARLRVLRDKLWNGLRDLEQVFLNGHETYNVANYLNVSFNFVEGESLMMALKDAAVSSGSACTSATLEPSYVLRALGLSDELAHSSIRFSFGKYTTEEDIDHVLEVTKAAVEKLRELSPLWDMYKEGIDLSTVEWAEH

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MODX01000010
EMBL· GenBank· DDBJ
OIJ38048.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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