A0A1J0VJ03 · A0A1J0VJ03_9GAMM
- ProteinUrease subunit alpha
- GeneureC
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids567 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
Catalytic activity
- urea + 2 H2O + H+ = hydrogencarbonate + 2 NH4+
Cofactor
Note: Binds 2 nickel ions per subunit.
Pathway
Nitrogen metabolism; urea degradation; CO2 and NH3 from urea (urease route): step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 133 | Ni2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 135 | Ni2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 216 | Ni2+ 1 (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 216 | Ni2+ 2 (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 218 | substrate | |||
Binding site | 245 | Ni2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 271 | Ni2+ 2 (UniProtKB | ChEBI) | |||
Active site | 319 | Proton donor | |||
Binding site | 359 | Ni2+ 1 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | nickel cation binding | |
Molecular Function | urease activity | |
Biological Process | urea catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUrease subunit alpha
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Oceanospirillales > Halomonadaceae > Halomonas
Accessions
- Primary accessionA0A1J0VJ03
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 216 | N6-carboxylysine | |||
Post-translational modification
Carbamylation allows a single lysine to coordinate two nickel ions.
Carboxylation allows a single lysine to coordinate two nickel ions.
Interaction
Subunit
Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 128-567 | Urease | |||
Sequence similarities
Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length567
- Mass (Da)60,704
- Last updated2017-02-15 v1
- MD5 ChecksumBF2CE80B2BF3FCE6C60EA144A77D749E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP018139 EMBL· GenBank· DDBJ | APE32016.1 EMBL· GenBank· DDBJ | Genomic DNA |