A0A1J0VBR9 · A0A1J0VBR9_9GAMM
- Protein7-carboxy-7-deazaguanine synthase
- GenequeE
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids218 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.
Catalytic activity
- 6-carboxy-5,6,7,8-tetrahydropterin + H+ = 7-carboxy-7-deazaguanine + NH4+
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 S-adenosyl-L-methionine per subunit.
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway
Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 11-13 | substrate | |||
Binding site | 26 | substrate | |||
Binding site | 30 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 45 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 47-49 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 48 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 50 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 91 | substrate | |||
Binding site | 93 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 134-136 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 174-177 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | carbon-nitrogen lyase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | S-adenosyl-L-methionine binding | |
Biological Process | queuosine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name7-carboxy-7-deazaguanine synthase
- EC number
- Short namesCDG synthase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Oceanospirillales > Halomonadaceae > Halomonas
Accessions
- Primary accessionA0A1J0VBR9
Proteomes
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 17-218 | Radical SAM core | |||
Sequence similarities
Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length218
- Mass (Da)23,171
- Last updated2017-02-15 v1
- Checksum19E43D68A1438CF5
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP018139 EMBL· GenBank· DDBJ | APE29496.1 EMBL· GenBank· DDBJ | Genomic DNA |