A0A1I5Q3Q8 · A0A1I5Q3Q8_9EURY

  • Protein
    Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ ion per subunit.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site105Fe cation (UniProtKB | ChEBI)
Binding site109Fe cation (UniProtKB | ChEBI)
Binding site126-130L-threonylcarbamoyladenylate (UniProtKB | ChEBI)
Binding site158L-threonylcarbamoyladenylate (UniProtKB | ChEBI)
Binding site171L-threonylcarbamoyladenylate (UniProtKB | ChEBI)
Binding site175L-threonylcarbamoyladenylate (UniProtKB | ChEBI)
Binding site271L-threonylcarbamoyladenylate (UniProtKB | ChEBI)
Binding site299Fe cation (UniProtKB | ChEBI)
Binding site362-370ATP (UniProtKB | ChEBI)
Binding site379ATP (UniProtKB | ChEBI)
Active site468Proton acceptor; for kinase activity

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular ComponentEKC/KEOPS complex
Molecular FunctionATP binding
Molecular Functioniron ion binding
Molecular Functionmetalloendopeptidase activity
Molecular FunctionN(6)-L-threonylcarbamoyladenine synthase activity
Molecular Functionprotein serine kinase activity
Molecular Functionprotein serine/threonine kinase activity
Molecular Functionprotein serine/threonine/tyrosine kinase activity
Molecular Functionzinc ion binding
Biological ProcesstRNA threonylcarbamoyladenosine modification

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including 2 domains:

  • Recommended name
    tRNA N6-adenosine threonylcarbamoyltransferase
  • EC number
  • Alternative names
    • tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
    • t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
  • Recommended name
    Serine/threonine-protein kinase Bud32
  • EC number

Gene names

    • ORF names
      SAMN05216277_103239

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • CGMCC 1.10329
  • Taxonomic lineage
    Archaea > Euryarchaeota > Stenosarchaea group > Halobacteria > Halobacteriales > Haloferacaceae

Accessions

  • Primary accession
    A0A1I5Q3Q8

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-338Kae1
Domain26-305Gcp-like
Region211-230Disordered

Sequence similarities

In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.
In the N-terminal section; belongs to the KAE1 / TsaD family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    548
  • Mass (Da)
    58,200
  • Last updated
    2017-04-12 v1
  • Checksum
    645A0FD3E4E86C1F
MRVLGVEGTAWCASAAVHDTETDETFIESDAYEPESGGIHPREAAEHMGDAVPRVIETALARADGDIDVVAFSKGPGLGPCLRVAGTAARALAGTLDVPLVGVNHMVAHLEIGRHESGFDSPVCLNASGANAHLLGFHDGRYRVLGETMDTGVGNAIDKFTRHLGWTHPGGPKVEEAAKDGEYVDLPYVVKGMDFSFSGLMSAAKDAVDDVSASEASGGPSAHRSDDGTPVEDVCFSLQEHVFAMLTEVSERALSLTGSDELVLGGGVGQNARLREMLAAMCEQRGAEFYAPEPRFLRDNAGMIAVLGAKMAAAGDTIAIADSAIDPDFRPDEVPVTWRANESVAVGHGVTPGTSMSGLSDPRRGAEAVVDVQDGVIVKRRIPKGYRHPDLDAKLRRDRTVLEARLFNEARKAGVPTPLVHDVDVPEATLRIQHVGDSDLADALTTDHARTLGQHLARLHGRGLVHGDPTTKNVRVGDRLFLVDFGLGYHSGHPEDHAMDLHVFEGSVAGTATDSAAICRAFEDGYAAVGDETVLERLAEVRGRGRYQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FOXI01000003
EMBL· GenBank· DDBJ
SFP40827.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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