A0A1I5MIP7 · A0A1I5MIP7_9PSED
- ProteinLactoylglutathione lyase
- GenegloA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids130 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
Catalytic activity
- (R)-S-lactoylglutathione = methylglyoxal + glutathione
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 nickel ion per subunit.
Note: Binds 1 zinc ion per subunit. In the homodimer, two zinc ions are bound between subunits.
Pathway
Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 56 | Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners | ||||
Sequence: E | ||||||
Binding site | 74 | Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners | ||||
Sequence: H | ||||||
Active site | 122 | Proton donor/acceptor | ||||
Sequence: E | ||||||
Binding site | 122 | Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | lactoylglutathione lyase activity | |
Molecular Function | metal ion binding | |
Biological Process | methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLactoylglutathione lyase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionA0A1I5MIP7
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 2-126 | VOC | ||||
Sequence: RLLHTMLRVGDLDQSIAFYTEVLGMTLLRRKDYPDGQFTLAFVGYGDEAHNSVIELTHNWGVDSYELGTGYGHIALEVEDVYKACEDIRSRGGKITREPGPMKHGSSILAFVEDPDGYKVELLSP |
Sequence similarities
Belongs to the glyoxalase I family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length130
- Mass (Da)14,579
- Last updated2017-04-12 v1
- Checksum69323D8559B3FA3F