A0A1I1K0C3 · A0A1I1K0C3_9GAMM
- ProteindITP/XTP pyrophosphatase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids204 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.
Catalytic activity
- H2O + ITP = diphosphate + H+ + IMP
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 11-16 | substrate | ||||
Sequence: SNNAGK | ||||||
Binding site | 43 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 72 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 72 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 73 | substrate | ||||
Sequence: S | ||||||
Binding site | 162-165 | substrate | ||||
Sequence: FGYD | ||||||
Binding site | 185 | substrate | ||||
Sequence: K | ||||||
Binding site | 190-191 | substrate | ||||
Sequence: HR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | dITP diphosphatase activity | |
Molecular Function | ITP diphosphatase activity | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotide binding | |
Molecular Function | ribonucleoside triphosphate phosphatase activity | |
Molecular Function | XTP diphosphatase activity | |
Biological Process | nucleotide metabolic process | |
Biological Process | purine nucleoside triphosphate catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namedITP/XTP pyrophosphatase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Oceanospirillales > Halomonadaceae > Kushneria
Accessions
- Primary accessionA0A1I1K0C3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Structure
Sequence
- Sequence statusComplete
- Length204
- Mass (Da)21,814
- Last updated2017-11-22 v1
- Checksum8F7CAE47634A065C