A0A1H7LIM8 · A0A1H7LIM8_9BACL

  • Protein
    Probable dual-specificity RNA methyltransferase RlmN
  • Gene
    rlmN
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site91Proton acceptor
Binding site111[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site115[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site118[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site161-162S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site193S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site216-218S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site292S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site335S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Probable dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • ORF names
      SAMN05518856_106289

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • OK003
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Paenibacillaceae > Paenibacillus

Accessions

  • Primary accession
    A0A1H7LIM8

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain97-330Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    346
  • Mass (Da)
    39,252
  • Last updated
    2017-11-22 v1
  • Checksum
    E3552672DAE13809
MKPFIYDYSLEELQQWAVENGEPAFRGGQIFDWIYVKRVNDFSEMTNLSKALREKLTEQFEFVTLTEITKFESKDGTVKFLFGLHDDHAIETVIMKHNYGNSICVTTQVGCRIGCTFCASTLGGLKRNLTAGEIVAQVVQAQKILDERGERVSSIVIMGSGEPFENYEATMTFLRIMIHEKGLNIGQRHITVSTSGIVPNIYKFADEDTQINLAISIHAPNDALRSKLMPVNRRFPFDDVMESLRYYLAKTGRRITFEYALIGGVNDQPEHAAELAGVLKNMLCHVNLIPVNHVPERKYVRTSRSDIFNFQKILSEQGVNVTIRREQGHDIAAACGQLRAKHMELR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FOBD01000006
EMBL· GenBank· DDBJ
SEK98773.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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