A0A1H0NKS3 · SAND_SELRU

  • Protein
    S-adenosylmethionine-dependent nucleotide dehydratase
  • Gene
    vip6
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    3/5

Function

function

Expression of pVip6 in E.coli (strain MG1655) confers resistance to phages lambda, P1, SECphi6, SECphi8 and T7. Catalyzes the conversion of cytidine triphosphate (CTP) to 3'-deoxy-3',4'-didehydro-CTP (ddhCTP), probably via a SAM-dependent radical mechanism (PubMed:32937646).
The modified nucleotide represses transcription from T7 RNA polymerase-directed genes (possibly by acting as chain terminators), strongly suggesting these nucleotides block viral polymerase transcription (By similarity).

Miscellaneous

How this protein allows bacteria to resist viruses that do not encode their own RNA polymerase (such as lambda, P1) is unknown.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site13[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site17[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site20[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionlyase activity
Molecular Functionmetal ion binding
Biological Processdefense response to virus

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    S-adenosylmethionine-dependent nucleotide dehydratase
  • EC number
  • Short names
    SAND
  • Alternative names
    • Prokaryotic viperin protein pVip6
      (pVip6
      )

Gene names

    • Name
      vip6
    • ORF names
      Ga0066891_103114, SAMN05216366_103114

Organism names

  • Taxonomic identifier
  • Strain
    • S137
  • Taxonomic lineage
    Bacteria > Bacillota > Negativicutes > Selenomonadales > Selenomonadaceae > Selenomonas

Accessions

  • Primary accession
    A0A1H0NKS3

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004564171-274S-adenosylmethionine-dependent nucleotide dehydratase

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-215Radical SAM core

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    274
  • Mass (Da)
    30,933
  • Last updated
    2017-04-12 v1
  • Checksum
    F3694907EEEFB6A4
MAYKVNLHITQKCNYACKYCFAHFDHHNDLTLGQWKHIIDNLKTSGLVDAINFAGGEPVLHRDFAAIVNYAYDQGFKLSIITNGSLMLNPKLMPPELFAKFDTLGISVDSINPKTLIALGACNNSQEVLSYDKLSHLITLARSVNPTIRIKLNTVITNLNADEDLTIIGQELDIARWKMLRMKLFIHEGFNNAPLLVSQADFDGFVERHAEVSHDIVPENDLTRSYIMVDNQGRLLDDETEEYKVVGSLLAEDFGTVFDRYHFDEATYASRYAG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FNJQ01000003
EMBL· GenBank· DDBJ
SDO93241.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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