A0A1G6LAC0 · A0A1G6LAC0_9BACT
- ProteinMultifunctional fusion protein
- GenequeH
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids378 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.
Catalytic activity
- epoxyqueuosine34 in tRNA + AH2 = queuosine34 in tRNA + A + H2O
Cofactor
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )
Note: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.
Pathway
tRNA modification; tRNA-queuosine biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 7 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 8 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 97 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 196 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 197 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 274 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 277 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | epoxyqueuosine reductase activity | |
Molecular Function | manganese ion binding | |
Molecular Function | RNA binding | |
Molecular Function | RNA-DNA hybrid ribonuclease activity | |
Biological Process | queuosine biosynthetic process | |
Biological Process | RNA catabolic process | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMultifunctional fusion protein
Including 2 domains:
- Recommended nameRibonuclease HII
- EC number
- Short namesRNase HII
- Recommended nameEpoxyqueuosine reductase QueH
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Campylobacterota > Desulfurellia > Desulfurellales > Desulfurellaceae > Desulfurella
Accessions
- Primary accessionA0A1G6LAC0
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for disulfide bond.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Disulfide bond | 355↔357 | Redox-active | |||
Keywords
- PTM
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length378
- Mass (Da)43,914
- Last updated2017-11-22 v1
- MD5 Checksum1FD62932C98722D38DB2FED95C390E6C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FMYU01000005 EMBL· GenBank· DDBJ | SDC40141.1 EMBL· GenBank· DDBJ | Genomic DNA |