A0A1G6LAC0 · A0A1G6LAC0_9BACT

  • Protein
    Multifunctional fusion protein
  • Gene
    queH
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.

Pathway

tRNA modification; tRNA-queuosine biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site7a divalent metal cation (UniProtKB | ChEBI)
Binding site8a divalent metal cation (UniProtKB | ChEBI)
Binding site97a divalent metal cation (UniProtKB | ChEBI)
Binding site196[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site197[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site274[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site277[4Fe-4S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionepoxyqueuosine reductase activity
Molecular Functionmanganese ion binding
Molecular FunctionRNA binding
Molecular FunctionRNA-DNA hybrid ribonuclease activity
Biological Processqueuosine biosynthetic process
Biological ProcessRNA catabolic process
Biological ProcesstRNA modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Multifunctional fusion protein

Including 2 domains:

  • Recommended name
    Ribonuclease HII
  • EC number
  • Short names
    RNase HII
  • Recommended name
    Epoxyqueuosine reductase QueH
  • EC number
  • Alternative names
    • Queuosine biosynthesis protein QueH

Gene names

    • Name
      queH
    • Synonyms
      rnhB
    • ORF names
      SAMN05660835_00760

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • DSM 8415
  • Taxonomic lineage
    Bacteria > Campylobacterota > Desulfurellia > Desulfurellales > Desulfurellaceae > Desulfurella

Accessions

  • Primary accession
    A0A1G6LAC0

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond355↔357Redox-active

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-188RNase H type-2

Sequence similarities

Belongs to the QueH family.
Belongs to the RNase HII family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    378
  • Mass (Da)
    43,914
  • Last updated
    2017-11-22 v1
  • MD5 Checksum
    1FD62932C98722D38DB2FED95C390E6C
MLIAGVDEAGRGPWAGPIVSAVCILPKKLNLIVKDSKVLTQKKREEIYKILTRHSIYGIGIATCKEIDRFGIVKAFELSIKRALLFMPKKPDLLLIDGKDHLNLPVKFKSIIDGDSKIACISAASILAKVSRDHHMILMSQKYPNYAFDQHKGYGTKKHIELLEKFGICDIHRKSFEPIKQFINGTFKPKLLLHACCAPCATSVIERLKEKFRLSIYFFNPNIQPKKEYQLRLKEMIELCKYHNLELIVPEYDPKEWFKKVKLYKNYPEGSKRCTVCYAYRLYKTAVFAKKHNFEYFTTTLSVSPLKKYNTILHIGKTLESLNVLFFEEDFKKKDGFKRSCELSKEFNFYRQNYCGCVYSYHESINRKLKKQAQSNAN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FMYU01000005
EMBL· GenBank· DDBJ
SDC40141.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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