A0A1G2XLD4 · A0A1G2XLD4_9BACT
- ProteinAminodeoxyfutalosine synthase
- GenemqnE
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids373 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Radical SAM enzyme that catalyzes the addition of the adenosyl radical to the double bond of 3-[(1-carboxyvinyl)oxy]benzoate, leading to aminodeoxyfutalosine (AFL), a key intermediate in the formation of menaquinone (MK, vitamin K2) from chorismate.
Catalytic activity
- 3-[(1-carboxyvinyl)-oxy]benzoate + H2O + S-adenosyl-L-methionine = 6-amino-6-deoxyfutalosine + H+ + hydrogencarbonate + L-methionine
Cofactor
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway
Cofactor biosynthesis; coenzyme F0 biosynthesis.
Quinol/quinone metabolism; menaquinone biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase activity | |
Molecular Function | 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity | |
Molecular Function | aminodeoxyfutalosine synthase activity | |
Molecular Function | iron ion binding | |
Biological Process | menaquinone biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAminodeoxyfutalosine synthase
- EC number
- Short namesAFL synthase ; Aminofutalosine synthase
- Alternative names
Gene names
Organism names
- Taxonomic lineageBacteria > Planctomycetota
Accessions
- Primary accessionA0A1G2XLD4
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 56-289 | Radical SAM core | ||||
Sequence: AYYIINRHINYSNICKNRCKFCAFSRDKEEAGSYEMDMEEILEKAIAAAEEGATELHIVGGLHPDLPFDFYVKMIGEIHERCPDVHIQAFTAVEVEHLSQMAKLPVRQTLKILKEAGLGSLPGGGAEVFSPRIREQLCPEKLSGEGWLRVMREAHKLGLRSNATMLYGHVETPEDRINHLIKLRELQDETGGFMSFIPLAFHPKNTELSNHSGTTAMLDLKVISISRLMLDNFD |
Sequence similarities
Belongs to the radical SAM superfamily. MqnE family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length373
- Mass (Da)42,133
- Last updated2017-02-15 v1
- ChecksumCCFAA6984A3C233C