A0A1G1D2T8 · A0A1G1D2T8_9BACT

Function

function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site13[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site49[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site83[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site157[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site161[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site164[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular Functionmethylthiotransferase activity
Biological ProcesstRNA methylthiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
  • EC number
  • Alternative names
    • (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
    • tRNA-i(6)A37 methylthiotransferase

Gene names

    • Name
      miaB
    • ORF names
      A2Z59_13080

Organism names

Accessions

  • Primary accession
    A0A1G1D2T8

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain4-120MTTase N-terminal
Domain143-373Radical SAM core
Domain376-444TRAM

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    452
  • Mass (Da)
    50,856
  • Last updated
    2017-02-15 v1
  • Checksum
    1D8A7399BDD72C15
MPEKNLALLTFGCQMNKYDSEKIAGILQKSGIRLTDDLDNADIVILNTCSIREKAEQKVYSQIGRLTPLKKKNSKLIIGVGGCVAQIQGEKILQRAPHVDLVFGTLNIYKLPHLIREVEEKGERIADIDNDYSEDNDLAPVKREGSIHAWLSIMHGCDNYCTYCVVPYTRGREKSRPKESILSEIRGLAEEGFKEVTLLGQNVNSYGRDTGEDIDFPDLLSIIDRVKGIKRIRFITSHPKDLSDKLIKVMADLPKVCEYLHLPIQAGSDRVLTDMNRGYTSGDYLKKIDRLKESIPEIALSTDIIIGFPGETDGDFEKTRDIVKKVCYDGIYLFKYSPRKETPAAIFSNQLSDTVKRARFEEILKLQKEITLKKNKELEGKVVEVLVEGEDKNANGKLIGRTRTNKIVNFSIDMPEDRKSLIGELVNVSILKSNLYSLEGILINIKGGGKYA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MHDL01000180
EMBL· GenBank· DDBJ
OGW01475.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help