A0A1F6YI10 · A0A1F6YI10_9BACT
- ProteintRNA-specific 2-thiouridylase MnmA
- GenemnmA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids400 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.
Catalytic activity
- uridine34 in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 2-thiouridine34 in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H+
RHEA-COMP:11727 CHEBI:65315
Position: 34+ RHEA-COMP:11726 + CHEBI:17499 + CHEBI:30616 = RHEA-COMP:11728 CHEBI:87170
Position: 34+ RHEA-COMP:10131 + CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 9-16 | ATP (UniProtKB | ChEBI) | |||
Binding site | 35 | ATP (UniProtKB | ChEBI) | |||
Active site | 98 | Nucleophile | |||
Binding site | 122 | ATP (UniProtKB | ChEBI) | |||
Site | 123 | Interaction with tRNA | |||
Active site | 222 | Cysteine persulfide intermediate | |||
Site | 382 | Interaction with tRNA | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uridine 2-sulfurtransferase activity | |
Biological Process | tRNA wobble position uridine thiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-specific 2-thiouridylase MnmA
- EC number
Gene names
Organism names
- Taxonomic lineageBacteria > Candidatus Nomurabacteria
Accessions
- Primary accessionA0A1F6YI10
Proteomes
Subcellular Location
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 93-95 | Interaction with target base in tRNA | |||
Region | 172-174 | Interaction with tRNA | |||
Domain | 230-297 | tRNA-specific 2-thiouridylase MnmA-like central | |||
Domain | 326-398 | tRNA-specific 2-thiouridylase MnmA-like C-terminal | |||
Region | 350-351 | Interaction with tRNA | |||
Sequence similarities
Belongs to the MnmA/TRMU family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length400
- Mass (Da)44,203
- Last updated2017-02-15 v1
- MD5 ChecksumD81ADF9DA7355B168DDA4A6EEF38B797
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
MFVW01000027 EMBL· GenBank· DDBJ | OGJ05957.1 EMBL· GenBank· DDBJ | Genomic DNA |