A0A1F6YI10 · A0A1F6YI10_9BACT

Function

function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Features

Showing features for binding site, active site, site.

140050100150200250300350400
Type
IDPosition(s)Description
Binding site9-16ATP (UniProtKB | ChEBI)
Binding site35ATP (UniProtKB | ChEBI)
Active site98Nucleophile
Binding site122ATP (UniProtKB | ChEBI)
Site123Interaction with tRNA
Active site222Cysteine persulfide intermediate
Site382Interaction with tRNA

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uridine 2-sulfurtransferase activity
Biological ProcesstRNA wobble position uridine thiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-specific 2-thiouridylase MnmA
  • EC number

Gene names

    • Name
      mnmA
    • ORF names
      A2192_02830

Organism names

Accessions

  • Primary accession
    A0A1F6YI10

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region93-95Interaction with target base in tRNA
Region172-174Interaction with tRNA
Domain230-297tRNA-specific 2-thiouridylase MnmA-like central
Domain326-398tRNA-specific 2-thiouridylase MnmA-like C-terminal
Region350-351Interaction with tRNA

Sequence similarities

Belongs to the MnmA/TRMU family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    400
  • Mass (Da)
    44,203
  • Last updated
    2017-02-15 v1
  • MD5 Checksum
    D81ADF9DA7355B168DDA4A6EEF38B797
MTKKVVFVGLSGGVDSAVSATLLKKSGYEVVGVFIKTWHPDFLVCNEEEERHDAMRVAAFLDIPFLTFDFESVYKKEVADYMISEYKAGRTPNPDVMCNKEVKFGAFFKKAISMGADYVATGHYAISNSWLASFGSLSPRARGALPLGPSACETPKKGKPAIFLYKGIDPSKDQSYFLWTLKQEQLSKILFPVGHLNKTEVRKLAKKFNLPVAEKKDSQGICFLGHVDLKEFLKHYIKEKKGKVLNVVGEEIGYHSGAVFNTLGERHGFTVTKKGPNDGAYYVVGKDIKKNILTVSQDKDLRNFPTFALGDIGQGTHGSLNFSNLCLALKDVNWISKVPESNKDYTAQIRYHGEFLLCVLKITGKTTAEVIFQKPVLVAPGQSCVIYDKDVCLGGGVVVQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MFVW01000027
EMBL· GenBank· DDBJ
OGJ05957.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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