Essential maintenance is planned to begin on Fri Jan 24 2025. The website may be temporarily unavailable. Please use our fallback: https://wwwdev.ebi.ac.uk/uniprot/front-end/fallback/ in case of any outage.

A0A1F5C6E7 · A0A1F5C6E7_9BACT

Function

function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ ion per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site159Fe cation (UniProtKB | ChEBI)
Binding site163Fe cation (UniProtKB | ChEBI)
Binding site193-197substrate
Binding site226substrate
Binding site239substrate
Binding site348substrate
Binding site378Fe cation (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functioniron ion binding
Molecular FunctionN(6)-L-threonylcarbamoyladenine synthase activity
Biological ProcesstRNA threonylcarbamoyladenosine modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA N6-adenosine threonylcarbamoyltransferase
  • EC number
  • Alternative names
    • N6-L-threonylcarbamoyladenine synthase
      (t(6)A synthase
      )
    • t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD
    • tRNA threonylcarbamoyladenosine biosynthesis protein TsaD

Gene names

    • Name
      tsaD
    • ORF names
      A3A25_00230

Organism names

Accessions

  • Primary accession
    A0A1F5C6E7

Proteomes

Subcellular Location

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain30-73Gcp-like
Domain115-384Gcp-like

Sequence similarities

Belongs to the KAE1 / TsaD family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    409
  • Mass (Da)
    45,360
  • Last updated
    2017-02-15 v1
  • MD5 Checksum
    85602D55A79BF1B22D00CF1C68AF52CA
MIILAIETSCDETAISVLKTSGGRRRPEFKILSNIVSSQVKVHAPFWGVVPNLAKREHQKNLPLVLIKALKEGRFPISNFQFPISKHSELKVQQIEKILEREPELLEQFKKSILSLKPPKIDIIAVTHGPGLEPALWVGVNFAKALGFFWKKPIIGINHLEGHALANWLAPVGKKSQFPISNFQKVFPALCLIVSGGHTQLVLMKDFGKYRTIGETRDDAAGEAFDKVARMLKLGYPGGPAIAKIASDIKNQKSPQLRSGQAKIKNIKLPRPMINSKDYDFSFSGLKTAVLYLIKDLEKNGYALNDFRAAIAAEFQQAVIDVLISKTLKAAENYKVKSVLLGGGVSANKNLRRQMEKAVKEKLPKVIYHEPGLKFTTDNAAMIAAAACFHLKRKKDWSKIETAANLRLG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MEYT01000041
EMBL· GenBank· DDBJ
OGD38438.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help