A0A1F0EV33 · A0A1F0EV33_9FIRM
- ProteinFumarate hydratase class II
- GenefumC
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids452 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.
Miscellaneous
There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.
Catalytic activity
- (S)-malate = fumarate + H2O
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 97-99 | substrate | |||
Binding site | 121-124 | substrate; in site B | |||
Binding site | 131-133 | substrate | |||
Binding site | 179 | substrate | |||
Active site | 180 | Proton donor/acceptor | |||
Active site | 310 | ||||
Binding site | 311 | substrate | |||
Binding site | 316-318 | substrate | |||
Site | 323 | Important for catalytic activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | fumarate hydratase activity | |
Biological Process | fumarate metabolic process | |
Biological Process | malate metabolic process | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFumarate hydratase class II
- EC number
- Short namesFumarase C
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Tissierellia > Tissierellales > Peptoniphilaceae > Anaerococcus
Accessions
- Primary accessionA0A1F0EV33
Proteomes
Subcellular Location
Interaction
Subunit
Homotetramer.
Structure
Family & Domains
Features
Showing features for domain, coiled coil.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 13-333 | Fumarate lyase N-terminal | |||
Coiled coil | 146-176 | ||||
Domain | 400-452 | Fumarase C C-terminal | |||
Sequence similarities
Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length452
- Mass (Da)50,812
- Last updated2017-02-15 v1
- MD5 Checksum9F3C98DA61A3CC12BFC1785E40E2480A
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LTSR01000093 EMBL· GenBank· DDBJ | OFO40251.1 EMBL· GenBank· DDBJ | Genomic DNA |