A0A1E4SSR3 · A0A1E4SSR3_9ASCO

Function

function

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.

Catalytic activity

Features

Showing features for binding site.

147050100150200250300350400450
TypeIDPosition(s)Description
Binding site238S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site276-277S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site304-305S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site363S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmitochondrial matrix
Cellular Componentnucleus
Molecular FunctiontRNA (guanine(37)-N1)-methyltransferase activity
Biological Processmitochondrial tRNA methylation
Biological ProcesstRNA N1-guanine methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA (guanine(37)-N1)-methyltransferase
  • EC number
  • Alternative names
    • M1G-methyltransferase
    • tRNA [GM37] methyltransferase
    • tRNA methyltransferase 5

Gene names

    • Name
      TRM5
    • ORF names
      CANARDRAFT_205009

Organism names

  • Taxonomic identifier
  • Strain
    • NRRL YB-2248
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Pichiaceae > Ogataea > Ogataea/Candida clade

Accessions

  • Primary accession
    A0A1E4SSR3

Proteomes

Subcellular Location

Mitochondrion matrix
Nucleus
Cytoplasm
Note: Predominantly in the mitochondria and in the nucleus.

Keywords

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain149-465SAM-dependent methyltransferase TRM5/TYW2-type
Region328-349Disordered

Sequence similarities

Belongs to the TRM5 / TYW2 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    470
  • Mass (Da)
    53,457
  • Last updated
    2017-01-18 v1
  • Checksum
    EEF023C6F2107C4A
MASTTPTHSLPFLTPEDLVPPVNRGLKVLDKSLFTKKFNVWSVTFPQFQHISDFLKNCKDDVLKLPKISPVTKPMPENNNMKSVLLTERLVDLSKKDQVLSKETLKDIDSNNGKFQLQSIELDYSYWKAEQILKAILPVELHDDVPSSFTVTGHLAHLNLREEFKPYDRLIGQVILDKNTSIKTVVDKVGTIDTTFRTFEMKVIAGKPDMIVELKESDCIFNFDFSKVYWNSRLSTEHNRIVSGFQAAEAVCDVMAGVGPFAVPAGKKKCLVFANDLNPNSFKYLSRNITKNKVQDYVVPFNQDGRDFIKSSPTILQQYALENPEVKLSKPKSSTHKNGTSKRHCPPTPTAPTPTFFSHYVMNLPDSAITFVDSYIGLFSNAFPQLTKEQIKELPGFKLPIINVHHFEKFEHTETPTDEELHKRVHSKLTKLLNYDIPFDNVKFHLVRMVAPTKPMYCVSFELPEEVAFA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KV453881
EMBL· GenBank· DDBJ
ODV82558.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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