A0A1E4SSR3 · A0A1E4SSR3_9ASCO
- ProteintRNA (guanine(37)-N1)-methyltransferase
- GeneTRM5
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids470 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Catalytic activity
- guanosine37 in tRNA + S-adenosyl-L-methionine = H+ + N1-methylguanosine37 in tRNA + S-adenosyl-L-homocysteine
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 238 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 276-277 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DL | ||||||
Binding site | 304-305 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DG | ||||||
Binding site | 363 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial matrix | |
Cellular Component | nucleus | |
Molecular Function | tRNA (guanine(37)-N1)-methyltransferase activity | |
Biological Process | mitochondrial tRNA methylation | |
Biological Process | tRNA N1-guanine methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (guanine(37)-N1)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Pichiaceae > Ogataea > Ogataea/Candida clade
Accessions
- Primary accessionA0A1E4SSR3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Predominantly in the mitochondria and in the nucleus.
Keywords
- Cellular component
Interaction
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 149-465 | SAM-dependent methyltransferase TRM5/TYW2-type | ||||
Sequence: FTVTGHLAHLNLREEFKPYDRLIGQVILDKNTSIKTVVDKVGTIDTTFRTFEMKVIAGKPDMIVELKESDCIFNFDFSKVYWNSRLSTEHNRIVSGFQAAEAVCDVMAGVGPFAVPAGKKKCLVFANDLNPNSFKYLSRNITKNKVQDYVVPFNQDGRDFIKSSPTILQQYALENPEVKLSKPKSSTHKNGTSKRHCPPTPTAPTPTFFSHYVMNLPDSAITFVDSYIGLFSNAFPQLTKEQIKELPGFKLPIINVHHFEKFEHTETPTDEELHKRVHSKLTKLLNYDIPFDNVKFHLVRMVAPTKPMYCVSFELPE | ||||||
Region | 328-349 | Disordered | ||||
Sequence: LSKPKSSTHKNGTSKRHCPPTP |
Sequence similarities
Belongs to the TRM5 / TYW2 family.
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length470
- Mass (Da)53,457
- Last updated2017-01-18 v1
- ChecksumEEF023C6F2107C4A
Keywords
- Technical term