A0A1E3V0E0 · A0A1E3V0E0_9GAMM
- ProteintRNA-dihydrouridine(16) synthase
- GenedusC
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids315 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U16 in tRNAs.
Catalytic activity
- 5,6-dihydrouridine16 in tRNA + NAD+ = uridine16 in tRNA + NADH + H+
- 5,6-dihydrouridine16 in tRNA + NADP+ = uridine16 in tRNA + NADPH + H+
Cofactor
Features
Showing features for site, binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Site | 35 | Interacts with tRNA; defines subfamily-specific binding signature | |||
Binding site | 68 | FMN (UniProtKB | ChEBI) | |||
Site | 95 | Interacts with tRNA | |||
Active site | 98 | Proton donor | |||
Binding site | 139 | FMN (UniProtKB | ChEBI) | |||
Binding site | 168 | FMN (UniProtKB | ChEBI) | |||
Site | 176 | Interacts with tRNA | |||
Binding site | 199-201 | FMN (UniProtKB | ChEBI) | |||
Binding site | 223-224 | FMN (UniProtKB | ChEBI) | |||
Site | 271 | Interacts with tRNA; defines subfamily-specific binding signature | |||
Site | 273 | Interacts with tRNA; defines subfamily-specific binding signature | |||
Site | 278 | Interacts with tRNA | |||
Site | 294 | Interacts with tRNA; defines subfamily-specific binding signature | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-dihydrouridine16 synthase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-dihydrouridine(16) synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Alteromonadales > Shewanellaceae > Shewanella
Accessions
- Primary accessionA0A1E3V0E0
Proteomes
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 4-297 | DUS-like FMN-binding | |||
Sequence similarities
Belongs to the Dus family. DusC subfamily.
Belongs to the dus family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length315
- Mass (Da)35,450
- Last updated2017-01-18 v1
- Checksum3E6A0E38D03A781F
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LDOA01000001 EMBL· GenBank· DDBJ | ODR86895.1 EMBL· GenBank· DDBJ | Genomic DNA |