A0A1D6EPW5 · A0A1D6EPW5_MAIZE
- ProteinATP-dependent 6-phosphofructokinase
- Gene103647427
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids524 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity
- beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H+
Cofactor
Activity regulation
Allosterically activated by AMP.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 176 | ATP (UniProtKB | ChEBI) | |||
Binding site | 240-241 | ATP (UniProtKB | ChEBI) | |||
Binding site | 265-268 | ATP (UniProtKB | ChEBI) | |||
Binding site | 266 | Mg2+ (UniProtKB | ChEBI); catalytic | |||
Site | 267 | Important for substrate specificity; cannot use PPi as phosphoryl donor | |||
Binding site | 294-296 | substrate | |||
Active site | 296 | Proton acceptor | |||
Binding site | 339-341 | substrate | |||
Binding site | 395 | substrate | |||
Binding site | 447-450 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Biological Process | fructose 6-phosphate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent 6-phosphofructokinase
- EC number
- Short namesATP-PFK ; Phosphofructokinase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > PACMAD clade > Panicoideae > Andropogonodae > Andropogoneae > Tripsacinae > Zea
Accessions
- Primary accessionA0A1D6EPW5
Proteomes
Genome annotation databases
Subcellular Location
Interaction
Subunit
Homotetramer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 169-471 | Phosphofructokinase | |||
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length524
- Mass (Da)57,690
- Last updated2016-11-30 v1
- Checksum3CC2708529E13522
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1D6EPW1 | A0A1D6EPW1_MAIZE | 103647427 | 536 | ||
A0A1D6EPW8 | A0A1D6EPW8_MAIZE | 103647427 | 405 | ||
A0A096S3M8 | A0A096S3M8_MAIZE | 103647427 | 527 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM007648 EMBL· GenBank· DDBJ | ONM21808.1 EMBL· GenBank· DDBJ | Genomic DNA |