A0A1C9U5X5 · RNMT_PAPSO
- ProteinReticuline N-methyltransferase
- GeneRNMT
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids363 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
N-methyltransferase accepting primarily 1-benzylisoquinoline and aporphine substrates possessing a tertiary amine. The preferred substrates are (R)- and (S)-reticuline and (S)-corytuberine, but can also use (R,S)-tetrahydropapaverine, papaverine, +-bulbocapnine, +-glaucine, +-isocorydine, +-isothebaine, boldine, noscapine, narcotine hemiacetal and hydrastine as substrates. Involved in the biosynthesis of magnoflorine.
Catalytic activity
- (S)-reticuline + S-adenosyl-L-methionine = (S)-tembetarine + H+ + S-adenosyl-L-homocysteine
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
42 μM | (S)-reticuline | |||||
85 μM | (R)-reticuline | |||||
168 μM | S-adenosyl-L-methionine |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
39.6 pmol/min/ug | with (S)-reticuline as substrate | ||||
74.8 pmol/min/ug | with (R)-reticuline as substrate | ||||
66.5 pmol/min/ug | toward S-adenosyl-L-methionine |
kcat is 0.028 sec-1 with (S)-reticuline as substrate. kcat is 0.053 sec-1 with (R)-reticuline as substrate. kcat is 0.047 sec-1 with S-adenosyl-L-methionine as substrate.
pH Dependence
Optimum pH is 8.5 with (R)-reticuline as substrate. Optimum pH is 7.0-9.0 with (S)-reticuline as substrate.
Temperature Dependence
Optimum temperature is 30 degrees Celsius.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | S-adenosyl-L-methionine:(S)-corytuberine-N-methyltransferase activity | |
Biological Process | methylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameReticuline N-methyltransferase
- EC number
- Short namesRNMT
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Ranunculales > Papaveraceae > Papaveroideae > Papaver
Accessions
- Primary accessionA0A1C9U5X5
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000439849 | 1-363 | Reticuline N-methyltransferase | |||
Sequence: MSTTMETTKISQQDDLWKNMELGQISDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLDFNKSLRGMKMATEIDTLENHKIYETPESFNQIIGGKESAGLFTDETTTTMEEANTKMMDLYCERAGLKDGHTILDLGCGAGLLVLHLAKKYKKSKITGITNTSSHKEYILKQCKNLNLSNVEIILADVTKVDIESTFDRVFVIGLIEHMKNFELFLRKISKWMKDDGLLLLEHLCHKSFSDHWEPLSEDDWYAKNFFPSGTLVIPSATCLLYFQEDVTVIDHWILSGNNFARSNEVILKRIDGKIEEVKDIFMSFYGIGREEAVKLINWWRLLCITANELFKYNNGEEWLISQLLFKKKLMTCI |
Expression
Tissue specificity
Expressed in roots and stems. Detected in leaves and flower buds.
Structure
Sequence
- Sequence statusComplete
- Length363
- Mass (Da)41,960
- Last updated2016-11-30 v1
- ChecksumB6327BAA0BEDD852