A0A1B9VKT8 · A0A1B9VKT8_9PROT

  • Protein
    Bifunctional enzyme IspD/IspF
  • Gene
    ispDF
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

a divalent metal cation (UniProtKB | Rhea| CHEBI:60240 )

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Features

Showing features for site, binding site.

TypeIDPosition(s)Description
Site17Transition state stabilizer
Site24Transition state stabilizer
Site154Positions MEP for the nucleophilic attack
Site208Positions MEP for the nucleophilic attack
Binding site246a divalent metal cation (UniProtKB | ChEBI)
Binding site246-2484-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site248a divalent metal cation (UniProtKB | ChEBI)
Site272Transition state stabilizer
Binding site272-2734-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site280a divalent metal cation (UniProtKB | ChEBI)
Binding site294-2964-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site370-3734-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Site371Transition state stabilizer
Binding site3774-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site3804-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
Molecular Function2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity
Molecular Functionmetal ion binding
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional enzyme IspD/IspF

Including 2 domains:

  • Recommended name
    2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
  • EC number
  • Alternative names
    • 4-diphosphocytidyl-2C-methyl-D-erythritol synthase
    • MEP cytidylyltransferase
      (MCT
      )
  • Recommended name
    2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
  • EC number
  • Short names
    MECDP-synthase
    ; MECPP-synthase
    ; MECPS

Gene names

    • Name
      ispDF
    • ORF names
      KO164_3229

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • KO164
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Rhodospirillales > Thalassospiraceae > Thalassospira

Accessions

  • Primary accession
    A0A1B9VKT8

Proteomes

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-2342-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Domain239-3922-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Region240-3972-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Sequence similarities

Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.
Belongs to the IspF family.
In the C-terminal section; belongs to the IspF family.
In the N-terminal section; belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    397
  • Mass (Da)
    43,226
  • Last updated
    2016-11-02 v1
  • Checksum
    03BF9F365F24948F
MTKKIAVVIVAAGSGSRFGDPIPKQYHLLGAKTLLRHCIESFAKYVPSELIRVIYNPDHQDLYDNSVKGLSLDAPVPGGVTRQQSVLNGLRSLENENPDHVLIHDAARPGISDDVIDRVISALATYQGAIPTLPVLDTIKQTESTGIIRRTIPREQLHRAQTPQGFHFKTILDAHQRFEGHEFTDDAALLEQLGLDVVCVEGAGSNDKITRPDDIERARDWINPVPASEKDSDMTHEEFRAGTGFDVHRFAPGDHCILCGVKVPHSARLEGHSDADVGLHTLTDALLGSIGAGDIGQHFPPSDMKWKGAASDQFVLHAVNLIRERGGRIVNADITLICERPKIGPHTQAMREKVAEILGIEVDRVNVKATTTERLGFTGREEGIAAQAAVSVALPVK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MAJC01000002
EMBL· GenBank· DDBJ
OCK09050.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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