A0A1B7YCL6 · DPCHH_COLHI

Function

function

Short chain dehydrogenase/reductase; part of the gene cluster that mediates the biosynthesis of the diterpenoid pyrones higginsianins A and B (PubMed:32286350).
The first step of the pathway is the synthesis of the alpha-pyrone moiety by the polyketide synthase dpchA via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units and 2 methylations (Probable). The alpha-pyrone is then combined with geranylgeranyl pyrophosphate (GGPP) formed by the GGPP synthase dpchD through the action of the prenyltransferase dpchC to yield a linear alpha-pyrone diterpenoid (Probable). Subsequent steps in the diterpenoid pyrone biosynthetic pathway involve the decalin core formation, which is initiated by the epoxidation of the C10-C11 olefin by the FAD-dependent oxidoreductase dpchE, and is followed by a cyclization cascade catalyzed by the terpene cyclase dpchB (Probable). The short chain dehydrogenase/reductase dpchG then oxidizes the 8S hydroxy group to a ketone and the short chain dehydrogenase/reductase dpchH reduces the ketone to the 8R hydroxy group to yield higginsianin B (PubMed:32286350).
Finally, the FAD-dependent oxidoreductase dpchF converts higginsianin B into higginsianin A (PubMed:32286350).

Biotechnology

Diterpenoid pyrones display various biological activities and higginsianin A shows anti-HIV activity.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site72-80NAD+ (UniProtKB | ChEBI)
Binding site99-100NAD+ (UniProtKB | ChEBI)
Binding site118-120NAD+ (UniProtKB | ChEBI)
Active site275Proton acceptor
Binding site275-279NAD+ (UniProtKB | ChEBI)
Binding site308-310NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionoxidoreductase activity
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Short chain dehydrogenase/reductase dpchH
  • EC number
  • Alternative names
    • Diterpenoid pyrone biosynthesis cluster protein H

Gene names

    • Name
      dpchH
    • ORF names
      CH063_02829, CH63R_05479

Organism names

Accessions

  • Primary accession
    A0A1B7YCL6
  • Secondary accessions
    • H1VQB2

Proteomes

Organism-specific databases

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane51-71Helical

Keywords

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00004515531-401Short chain dehydrogenase/reductase dpchH
Glycosylation16N-linked (GlcNAc...) asparagine
Glycosylation242N-linked (GlcNAc...) asparagine
Glycosylation386N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Interaction

Protein-protein interaction databases

Family & Domains

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    401
  • Mass (Da)
    43,505
  • Last updated
    2016-11-02 v1
  • Checksum
    8D9FA383AE9D62D8
MWTGQPASGPPFDHENHSASCTQHFALFSSPSPANSEPKSMLSAWAHRLCVRAVDVLFGTFLYVPLGILFLKKSLSGFGDGDWDSSQIPDLHGKVAVVTGGNAGIGYHTVRQLAAKGAKVYLAARSESRAKEAIKRLREENPDIPQEKLVWLPLDLSSQAQVVDAARDLMSKTERLDILVNNAGVDPYNYVKTADGFEMTMAVNHIGHWTLTYCLLPLLKATAAQQGSDVRVITLSSSGERNHSANNHFTTLKDLDDPCAGPGWEDSRLAQGKRYGTSKLANILFATELQRRMDEEGAGILSLSLNPGTIRTEGAADVMPLVTQPLVWLLFTDAAKGADTTMFAATAREVRENSEQWKGRYLDGPGRIKPPSPKARDAVAARNLWNITAAAVKGTGALEKL

Sequence caution

The sequence CACQ02005368 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM004458
EMBL· GenBank· DDBJ
OBR09787.1
EMBL· GenBank· DDBJ
Genomic DNA
CACQ02005368
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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