A0A1B7YCK2 · DPCHC_COLHI

Function

function

Polyprenyl transferase; part of the gene cluster that mediates the biosynthesis of the diterpenoid pyrones higginsianins A and B (PubMed:32286350).
The first step of the pathway is the synthesis of the alpha-pyrone moiety by the polyketide synthase dpchA via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units and 2 methylations (Probable). The alpha-pyrone is then combined with geranylgeranyl pyrophosphate (GGPP) formed by the GGPP synthase dpchD through the action of the prenyltransferase dpchC to yield a linear alpha-pyrone diterpenoid (Probable). Subsequent steps in the diterpenoid pyrone biosynthetic pathway involve the decalin core formation, which is initiated by the epoxidation of the C10-C11 olefin by the FAD-dependent oxidoreductase dpchE, and is followed by a cyclization cascade catalyzed by the terpene cyclase dpchB (Probable). The short chain dehydrogenase/reductase dpchG then oxidizes the 8S hydroxy group to a ketone and the short chain dehydrogenase/reductase dpchH reduces the ketone to the 8R hydroxy group to yield higginsianin B (PubMed:32286350).
Finally, the FAD-dependent oxidoreductase dpchF converts higginsianin B into higginsianin A (PubMed:32286350).

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Biotechnology

Diterpenoid pyrones display various biological activities and higginsianin A shows anti-HIV activity.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functiontransferase activity, transferring alkyl or aryl (other than methyl) groups
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Polyprenyl transferase dpchC
  • EC number
  • Alternative names
    • Diterpenoid pyrone biosynthesis cluster protein C

Gene names

    • Name
      dpchC
    • ORF names
      CH063_02830, CH63R_05478

Organism names

Accessions

  • Primary accession
    A0A1B7YCK2
  • Secondary accessions
    • H1VQB3

Proteomes

Organism-specific databases

Subcellular Location

Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane24-44Helical
Transmembrane60-80Helical
Transmembrane105-125Helical
Transmembrane154-174Helical
Transmembrane192-212Helical
Transmembrane234-254Helical
Transmembrane258-278Helical
Transmembrane296-316Helical

Keywords

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004515331-316Polyprenyl transferase dpchC

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the UbiA prenyltransferase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    316
  • Mass (Da)
    35,448
  • Last updated
    2016-11-02 v1
  • Checksum
    9EA58F0555BFF751
MAATKDRQLFRHLLRLSRFDRYNPLFTTFAGLWSTLLAGGAKMAEQRSDMSVATVFQQCALCFVASYLFCGAGMVWNDWIDRDIDANVARTKERPLASGKVTATEAMVWMVLQAALSWGVLEVMLDGKDVAKHFIPVAAASVLYPFGKRSLARMLGIYPQYILAFTIAWPAVIGRAAIYGQYESYAETLRQCLPLCTMVFFWTIYLNTAYSYQDVVDDRKLGVNSFYNIAGKHIHLLLVALVSPILVCLPIYLFELHSLWLWLSWMGVWTASLAQQLVQFDPKQPASGGSIHRSNFILGIWTILACVVQVFLTGSA

Sequence caution

The sequence CACQ02005368 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LTAN01000004
EMBL· GenBank· DDBJ
OBR09786.1
EMBL· GenBank· DDBJ
Genomic DNA
CACQ02005368
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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