A0A1B7YCC9 · DPCHA_COLHI

Function

function

Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the diterpenoid pyrones higginsianins A and B (PubMed:32286350).
The first step of the pathway is the synthesis of the alpha-pyrone moiety by the polyketide synthase dpchA via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units and 2 methylations (Probable). The alpha-pyrone is then combined with geranylgeranyl pyrophosphate (GGPP) formed by the GGPP synthase dpchD through the action of the prenyltransferase dpchC to yield a linear alpha-pyrone diterpenoid (Probable). Subsequent steps in the diterpenoid pyrone biosynthetic pathway involve the decalin core formation, which is initiated by the epoxidation of the C10-C11 olefin by the FAD-dependent oxidoreductase dpchE, and is followed by a cyclization cascade catalyzed by the terpene cyclase dpchB (Probable). The short chain dehydrogenase/reductase dpchG then oxidizes the 8S hydroxy group to a ketone and the short chain dehydrogenase/reductase dpchH reduces the ketone to the 8R hydroxy group to yield higginsianin B (PubMed:32286350).
Finally, the FAD-dependent oxidoreductase dpchF converts higginsianin B into higginsianin A (PubMed:32286350).

Biotechnology

Diterpenoid pyrones display various biological activities and higginsianin A shows anti-HIV activity.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site567For beta-ketoacyl synthase activity
Active site703For beta-ketoacyl synthase activity
Active site745For beta-ketoacyl synthase activity
Active site1027For acyl/malonyl transferase activity

GO annotations

AspectTerm
Molecular Function3-oxoacyl-[acyl-carrier-protein] synthase activity
Molecular Functionmethyltransferase activity
Biological Processfatty acid biosynthetic process
Biological Processmethylation
Biological Processsecondary metabolite biosynthetic process
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Non-reducing polyketide synthase dpchA
  • EC number
  • Alternative names
    • Diterpenoid pyrone biosynthesis cluster protein A

Gene names

    • Name
      dpchA
    • ORF names
      CH063_11355, CH63R_05473

Organism names

Accessions

  • Primary accession
    A0A1B7YCC9
  • Secondary accessions
    • H1VL15

Proteomes

Organism-specific databases

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004515231-2224Non-reducing polyketide synthase dpchA
Modified residue1749O-(pantetheine 4'-phosphoryl)serine

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region74-183N-terminal acylcarrier protein transacylase domain (SAT)
Domain426-822Ketosynthase family 3 (KS3)
Region941-1245Malonyl-CoA:ACP transacylase (MAT) domain
Region1317-1451N-terminal hotdog fold
Domain1317-1626PKS/mFAS DH
Region1327-1620Product template (PT) domain
Region1476-1626C-terminal hotdog fold
Region1635-1664Disordered
Region1684-1717Disordered
Domain1713-1789Carrier
Region2022-2207Methyltransferase (CMeT) domain

Domain

Multidomain protein; including a starter unit:ACP transacylase (SAT) that selects the starter unit; a ketosynthase (KS) that catalyzes repeated decarboxylative condensation to elongate the polyketide backbone; a malonyl-CoA:ACP transacylase (MAT) that selects and transfers the extender unit malonyl-CoA; a product template (PT) domain that controls the immediate cyclization regioselectivity of the reactive polyketide backbone; a methyltransferase (CMeT) domain responsible for methylations; and an acyl-carrier protein (ACP) that serves as the tether of the growing and completed polyketide via its phosphopantetheinyl arm.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,224
  • Mass (Da)
    240,092
  • Last updated
    2016-11-02 v1
  • Checksum
    24ACD0E52A7883C3
MSAETPSLLVCGPLISDPDATHLARIRSALVHTPQLAELRQAVTELPGVWSLLAGGEPSLERVHAAPLLQAFAEWINKGNSSGLLAAGQSSRNTRLAVLTVLSHLAEYVTFLRGLDAGAEEDLAGELDEHTKTLGGLRDGGVQGLCVGMLSASALACARNTSEVAELGAVAVRLAMCSAAFVDLDQIQSSDPTVCVSARLPRNAGEGDEQDRQPFQEALESYPEVRIQHSQSSQSWVQKEKAWLTCALRRQAYIGVRMDVAGTTITATKSITGPLTRHLEGQGAMTKEIDLKGRFHYPGHEDALQKLVRLCESTPMLQFPQNRYPLVPLRQTITGDVVTDETPLHETVLRCILTETADWHTTMTRAVSALAGSPETKSSPAGNRTRLLVQLGIAECIPRSVLAGIPSIRVLQPTTGKPARPGHYPDDAIAVIGMSCRFPDAETPEHFWEIIQSGAKAGSVFPDVGSFDCGLFRKSPREAEYMDPQHRLALHLAYEALEAAGHFSPSSSSTDDVGCYVGMSSCDYEDHVNARPPTAFSFTGTARAFASGRISHFFGLTGPSVVVDTACSSSGVAIHTACRAVLSGECAVALAGGVNLMTDEGQAHQNLAGASFLSPTGQCRPFDAAADGYRRGQGGGFVLLKRLSAAVADNDRVLGVIAASAVNNSKGNRSITLPSSESQSRLYRQVLGSANVHPSHVSYVEAHGTGTQKGDPVECQSIRSVFGGSSRAGSSSPIRLGSVKGNLGHGEAASGIASLVKVLLMLQHGVITPQANFSMLNPAIPPLEGDNMEIVVSPASWRGPFRTALVNNYGASGANAAMVVCQAPSTHLSQIASTRTAAMTTTHRYPFVITANTASSLHRSCLALLRFIETLPAGLNNDSLPSLAFHLAQRQNHALVHRIVFSARSVAELKAHLLAQVEGGNIPSDTSSQAQKTGTKPVVLLFSGQTGRRAHLNRDAYTSFHLLRHHLDRCDRTLQTLGLRSLFPRIFDADPLEDLVDLHCMQFALQYSVAASWTDSGLEIKALVGHSLGQLTALCVSGALSLADSLRMVSGRALLIQTAWKQERGCMLSVDADAATVETLAQSVSAEDKVEIACYNAALHQVVAGTEAAVAAFENAAGSSGISTKRLAVTHAFHSKLLDDILPEYHRLLRSLEFRPAKIPIEPCSEFGGGWDSVTPDIVGRQSREPVYFAAAMARVEQRLGSCVWLEVGSGSAAITMARRALESQKPSSQAMVSHSFYAAQLHSPEPVSSVADSTVGLWNEGVRVQFWLYHASQRQSFVPMDLPSYQFDKTQYWLPFIERNKGSDSNDQSASSQAAPDLVTLVGSLGSAEPQAYEFSINQESDEYALFVKGRTVFGHFLAPGSVYAESAARAFALLPTDNTPQPPASVELGQMKLHAPFGLDLQRRLRLVLRQQTASSWEFVVESCPLHDDKEISPKPQASGTVRLQGQGRSPFGTSQSILRRLFDRCGELREDRGASVVQGAYVKKIMSRVASYDDRYFGIRFVASRGLEAVGDVDALPIVSECRAGTALSPPVFDNFLLVAEMHAGSLGDLADDHLYICGGFEAIVPGDQTSQTDGPWTVLSTLERENDKTLVSDILVFHAGSKKLALSILGARLTQIPARSLQKTLDDMNAARPAKVSNSEDVLTPPLPAVESPPTDLSNGQIHCDLRSALSPLIRSTTSLSQLTSSDDTKDSLGLSSRPSITPASSATSENDQDTTALYNLLAEHLDCSNGIPPDMPLGNIGLDSLISIQLQSDLEKLFGKSPALKLIDENTTFLELCGMVLQQDLSSQLKSRLSPVDSANARTTLNEPLQAYGGYGHAMSVVAPAPVSPQDTPPFLPLAVEAFKRVKKDTGAFAQKTGFAGFYPDVQQKQTSLVLSYILEAFATLGCDLGTLQLKDRLPGIPHPAKYQRLMGRLHDILEEAGIISPPDAQLFRYRTDAPLPPPTPSEDLYRQILDECPLYRPDHQLLGVTASRLADCISGRADPLQLLFQDQASLKLLEDVYVSSPMFSTGNSMLGEMLRGLFSQARFQREGGSEKLRILEIGAGTGATTRRVLDQLIQSGVDFSYTFTDISLALVNGSKRKFTASYGRQRVDSDMEFTVLDIERPPPASMLQAFHLVISSNCIHATRDLRTSCGNIEKLVRRGDGMLCLLELTRPLAWLDCVFGLLDGWWRFEDGRKYALAHEDEWRARLESAGFQNVDWTGDGSRESEHFRLITAWH

Sequence caution

The sequence CACQ02004382 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM004458
EMBL· GenBank· DDBJ
OBR09781.1
EMBL· GenBank· DDBJ
Genomic DNA
CACQ02004382
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

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