A0A1B4ZDK7 · A0A1B4ZDK7_9ACTN

  • Protein
    4-hydroxy-tetrahydrodipicolinate synthase
  • Gene
    dapA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).

Caution

Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.

Features

Showing features for site, binding site, active site.

TypeIDPosition(s)Description
Site53Part of a proton relay during catalysis
Binding site54pyruvate (UniProtKB | ChEBI)
Site116Part of a proton relay during catalysis
Active site142Proton donor/acceptor
Active site170Schiff-base intermediate with substrate
Binding site210pyruvate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4-hydroxy-tetrahydrodipicolinate synthase activity
Biological Processdiaminopimelate biosynthetic process
Biological Processlysine biosynthetic process via diaminopimelate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-tetrahydrodipicolinate synthase
  • EC number
  • Short names
    HTPA synthase

Gene names

    • Name
      dapA

Organism names

  • Taxonomic identifier
  • Strain
    • SANK 60404
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces

Accessions

  • Primary accession
    A0A1B4ZDK7

Subcellular Location

Keywords

Interaction

Subunit

Homotetramer; dimer of dimers.

Family & Domains

Sequence similarities

Belongs to the DapA family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    299
  • Mass (Da)
    31,312
  • Last updated
    2016-11-02 v1
  • Checksum
    017688E2DEBB4C83
MAPISTPQTPFGRVLTAMVTPFTADGALDVDGAQRLAAHLVDAGNDGLVVNGTTGESPTTSDAEKDQLVRAVLEAVGDRAHVVAGIGTNDTHHSVELARAAERAGAHGLLAVTPYYNKPPQEGLFRHFTAIADATGLPVMLYDIPGRSGVPIDTETIVRLAAHPRIVANKDAKGDLGRASWAIARSGLAWYSGDDMLNLPLLSVGACGFVSVVGHVVTPELRTLLDAHLTGDVQKATEVHQRLLPVFTGMFRTQGVITTKAALTLQGLPAGPLRLPLVELTPEETAQLKIDLAAGGVQL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LC163892
EMBL· GenBank· DDBJ
BAV57522.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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