A0A1B3DF32 · A0A1B3DF32_PSEFL

  • Protein
    Carbamoyl phosphate synthase small chain
  • Gene
    carA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site47L-glutamine (UniProtKB | ChEBI)
Binding site241L-glutamine (UniProtKB | ChEBI)
Binding site243L-glutamine (UniProtKB | ChEBI)
Active site269Nucleophile
Binding site270L-glutamine (UniProtKB | ChEBI)
Binding site273L-glutamine (UniProtKB | ChEBI)
Binding site311L-glutamine (UniProtKB | ChEBI)
Binding site313L-glutamine (UniProtKB | ChEBI)
Binding site314L-glutamine (UniProtKB | ChEBI)
Active site353
Active site355

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      IM720_27455

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • KF1
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    A0A1B3DF32

Proteomes

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-192CPSase
Domain3-133Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    378
  • Mass (Da)
    40,715
  • Last updated
    2016-11-02 v1
  • Checksum
    731E0529B4B20367
MTKPAILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQIVTLTYPHIGNTGTTPEDVESDRVWSAGLVIRDLPLVASNWRNTMSLSDYLKANNVVAIAGIDTRRLTRILREKGSQNGCIMAGDNISEEAAIAAAQGFPGLKGMDLAKVVSTKEQYEWRSTVWDLKTDSHATIDAADLSYHVVAYDYGIKYNILRMLVERGCRVTVVPAQTPASDVLALQPDGVFLSNGPGDPEPCDYAIKAIKDVLETDIPVFGICLGHQLLALASGAKTLKMGHGHHGANHPVQDLDTGVVMITSQNHGFAVDEATLPGNVRAIHKSLFDGSLQGIERTDKSAFSFQGHPEASPGPNDVAPLFDRFINEMAKRR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP063233
EMBL· GenBank· DDBJ
QOU04385.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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