A0A1B1B6G4 · A0A1B1B6G4_9ACTN

Function

function

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site149Nucleophile; methyl group acceptor

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionDNA binding
Molecular Functionmetal ion binding
Molecular Functionmethylated-DNA-[protein]-cysteine S-methyltransferase activity
Biological ProcessDNA alkylation repair
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Methylated-DNA--protein-cysteine methyltransferase
  • EC number
  • Alternative names
    • 6-O-methylguanine-DNA methyltransferase
      (MGMT
      )
    • O-6-methylguanine-DNA-alkyltransferase

Gene names

    • ORF names
      AVL59_36715

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 14511
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces

Accessions

  • Primary accession
    A0A1B1B6G4

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain12-91Methylguanine DNA methyltransferase ribonuclease-like
Domain98-178Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding

Sequence similarities

Belongs to the MGMT family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    184
  • Mass (Da)
    19,302
  • Last updated
    2016-11-02 v1
  • Checksum
    023A43D3F72E4BA9
MDSHGQDEQRVVWAVVGTGIGPLMLAATRDGLVNVVFHATDAVRERALERLAARLGTEPAEDPDSPLLAEAIRQVEAYFAGERHDFDLPLDWSLISGFNRQVLRELASGVPYGTVVGYGDLAGRVGQPGGAQAVGAAMGANPLPVVVPCHRVVESDGGIGGFGGGLEAKRKLLALEGVLPEPLF

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP016279
EMBL· GenBank· DDBJ
ANP54393.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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