A0A1B1B528 · A0A1B1B528_9ACTN

Function

function

The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single-stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.

Catalytic activity

  • Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).
    EC:3.1.21.10 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 Mg2+ ion per subunit.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site7
Binding site7Mg2+ 1 (UniProtKB | ChEBI)
Active site68
Binding site68Mg2+ 2 (UniProtKB | ChEBI)
Active site141
Binding site141Mg2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular ComponentHolliday junction resolvase complex
Molecular Functioncrossover junction DNA endonuclease activity
Molecular FunctionDNA binding
Molecular Functionmagnesium ion binding
Biological ProcessDNA recombination
Biological ProcessDNA repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Crossover junction endodeoxyribonuclease RuvC
  • EC number
  • Alternative names
    • Holliday junction nuclease RuvC
    • Holliday junction resolvase RuvC

Gene names

    • Name
      ruvC
    • ORF names
      AVL59_34090

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 14511
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces

Accessions

  • Primary accession
    A0A1B1B528

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer which binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA4-RuvB12-RuvC2 complex forms which resolves the HJ.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the RuvC family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    179
  • Mass (Da)
    18,782
  • Last updated
    2016-11-02 v1
  • Checksum
    850EAF6924EFA36C
MRVLGVDPGLTRCGIGVVEGIAGRPLTMLGVGVVRTPVDADLSLRLLAAEEGIEEWLDEHRPEVVAVERVFSQHNVRTVMGTAQVSAVAMLCAARRGIPVALHTPSEVKAAVTGSGRADKAQVGAMVTRLLRLAAPPKPADAADALALAICHIWRAPAQNRLQQAVALHTVNATKGRTA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP016279
EMBL· GenBank· DDBJ
ANP53925.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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