A0A1A9JKZ1 · A0A1A9JKZ1_9PSED

  • Protein
    Serine hydroxymethyltransferase
  • Gene
    glyA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.

Catalytic activity

Cofactor

pyridoxal 5'-phosphate (UniProtKB | Rhea| CHEBI:597326 )

Pathway

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.
One-carbon metabolism; tetrahydrofolate interconversion.

Features

Showing features for binding site, site.

Type
IDPosition(s)Description
Binding site121(6S)-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site125-127(6S)-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Site229Plays an important role in substrate specificity
Binding site246(6S)-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site355-357(6S)-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functioncobalt ion binding
Molecular Functionglycine hydroxymethyltransferase activity
Molecular Functionpyridoxal phosphate binding
Molecular Functionserine binding
Molecular Functionzinc ion binding
Biological Processfolic acid metabolic process
Biological Processglycine biosynthetic process from serine
Biological ProcessL-serine catabolic process
Biological Processtetrahydrofolate interconversion

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Serine hydroxymethyltransferase
  • EC number
  • Short names
    SHMT
    ; Serine methylase

Gene names

    • Name
      glyA
    • ORF names
      A8L59_24370
      , A9HBioS_0738

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • CRS05-R5
    • A9
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    A0A1A9JKZ1

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue230N6-(pyridoxal phosphate)lysine

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain10-386Serine hydroxymethyltransferase-like

Sequence similarities

Belongs to the SHMT family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    417
  • Mass (Da)
    44,668
  • Last updated
    2016-10-05 v1
  • Checksum
    3CE944A1D7D573FF
MFSKQDQIQGYDDALLAAMNAEEQRQEDHIELIASENYTSKRVMQAQGSGLTNKYAEGYPGKRYYGGCEHVDKVEALAIERAKQLFGADYANVQPHSGSSANSAVYLALIQPGDTILGMSLAHGGHLTHGAKVSSSGKLYNAVQYGINTDTGLIDYDEVERLAVECKPKMIVAGFSAYSKTLDFPRFRQIADKVGALLFVDMAHVAGLVAAGLYPNPLPYADVVTTTTHKTLRGPRGGLILAKSNEEIEKKLNAAVFPGAQGGPLMHVIAGKAVCFKEALEPGFKAYQQQVIDNAKAMASVFIKRGYDVVSGGTDNHLFLVSLIRQGLTGKDADAALGRAHITVNKNAVPNDPQSPFVTSGLRIGTPAVTTRGFKVAQCEVLAGWICDILDNLGDADVEANVAKNVAALCADYPVYR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP015852
EMBL· GenBank· DDBJ
ANI00412.1
EMBL· GenBank· DDBJ
Genomic DNA
MKWS01000002
EMBL· GenBank· DDBJ
RVD79093.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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