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A0A1A8VUF5 · A0A1A8VUF5_PLAOA

Function

function

Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.

Miscellaneous

Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 manganese ions per subunit.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site121Mn2+ 1 (UniProtKB | ChEBI)
Binding site124Mn2+ 2 (UniProtKB | ChEBI)
Binding site124Mn2+ 1 (UniProtKB | ChEBI)
Binding site228-232GMP (UniProtKB | ChEBI)
Binding site229Mn2+ 1 (UniProtKB | ChEBI)
Binding site261Mn2+ 2 (UniProtKB | ChEBI)
Binding site355Mn2+ 2 (UniProtKB | ChEBI)
Binding site355-356GMP (UniProtKB | ChEBI)
Binding site404-407GMP (UniProtKB | ChEBI)
Binding site411GMP (UniProtKB | ChEBI)
Active site430GMP-histidine intermediate
Binding site430-433GMP (UniProtKB | ChEBI)
Binding site506GMP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentnucleus
Cellular ComponenttRNA-splicing ligase complex
Molecular FunctionGTP binding
Molecular Functionmetal ion binding
Molecular FunctionRNA ligase (ATP) activity
Molecular FunctionRNA ligase (GTP) activity
Biological ProcesstRNA splicing, via endonucleolytic cleavage and ligation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA-splicing ligase RtcB homolog
  • EC number
  • Alternative names
    • 3'-phosphate/5'-hydroxy nucleic acid ligase

Gene names

    • ORF names
      POVCU1_018070
      , POVCU2_0020150

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Eukaryota > Sar > Alveolata > Apicomplexa > Aconoidasida > Haemosporida > Plasmodiidae > Plasmodium > Plasmodium (Plasmodium)

Accessions

  • Primary accession
    A0A1A8VUF5

Proteomes

Organism-specific databases

Subcellular Location

Interaction

Subunit

Catalytic component of the tRNA-splicing ligase complex.

Family & Domains

Sequence similarities

Belongs to the RtcB family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    507
  • Mass (Da)
    56,371
  • Last updated
    2016-10-05 v1
  • MD5 Checksum
    368056D12F58FE3CFE45F26A7379C6D2
MKTARKPDDVFGYIEKTKEQNLYRINKGLVTGMNVEGHMYVNERLKVLIDEEIRTYQSSKCSAFLPAVVQIANVSTLPGIVKASIALPDVHAGYGFSIGNVAAFDMSNEKAIVSPGGVGFDINCGVRLIRTNLFYDDIKEKQEQLTQLLFNHIPVGVGSQGFILCNQENLDDALCLGMDWCIKEGYSWIEDKLNCEDNGRSLYADSNYVSIRAKKRGITQMGTLGAGNHYAEIQIVDEIYNKRSAKLMGIEKKKQVCIMIHSGSRGLGHQIATDALIEMEKSMNKYKINVIDKQLACTPIHSKEGKNYLKAMGSACNFAWINRSSMTFLARQAFSKIFNQSPDDLDMHVIYDVSHNIAKIEQHVINGKLQNLLVHRKGSTRAFPPFHPLVPLDYQYCGQPILIGGTMGTYSYVLTGTEKAMQATFGSTCHGAGRALSRNKSRNTLSYVDVLNKLKEENISIRVASPKLIMEEAPESYKNVSEVVQTCHDAGISDKCFRLKPIAVIKG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FLQU01000265
EMBL· GenBank· DDBJ
SBS83311.1
EMBL· GenBank· DDBJ
Genomic DNA
FLQV01000332
EMBL· GenBank· DDBJ
SBS90287.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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