A0A1A8VSR8 · A0A1A8VSR8_9APIC
- ProteinPutative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids607 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.
Catalytic activity
- cytidine32/guanosine34 in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine32/2'-O-methylguanosine34 in tRNA + 2 H+ + 2 S-adenosyl-L-homocysteine
RHEA-COMP:10246 CHEBI:74269 Position: 34CHEBI:82748 Position: 32+ 2 CHEBI:59789 = RHEA-COMP:10247 CHEBI:74445 Position: 34CHEBI:74495 Position: 32+ 2 CHEBI:15378 + 2 CHEBI:57856
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 75 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 77 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 149 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 165 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 216 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 256 | Proton acceptor | ||||
Sequence: K |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | tRNA (cytidine(32)-2'-O)-methyltransferase activity | |
Molecular Function | tRNA (guanine(34)-2'-O)-methyltransferase activity | |
Biological Process | cytoplasmic translation | |
Biological Process | tRNA nucleoside ribose methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePutative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Sar > Alveolata > Apicomplexa > Aconoidasida > Haemosporida > Plasmodiidae > Plasmodium > Plasmodium (Plasmodium)
Accessions
- Primary accessionA0A1A8VSR8
Proteomes
Organism-specific databases
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain, compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 21-81 | Ribosomal RNA methyltransferase FtsJ | ||||
Sequence: YRARSSYKLIQINEKFEIFKLFNLNNYSNKDISDLIDIYNNRYCYNIVDLCAAPGSWSQVL | ||||||
Domain | 141-299 | Ribosomal RNA methyltransferase FtsJ | ||||
Sequence: REPKMVAVDLQEIGNMGYVQIIQGDITKGSTIDRILKCMEGGNACNEGNARCRKDGVSYLDRKDCRVHYAHAVVSDGAPDITGMNDIDEFIQSQLILSSLKVCCSVLKIGGNFISKIFRGEHTGLLILHLNKFFERVYVCKPQSSRNKSLESFLVCLNF | ||||||
Compositional bias | 354-371 | Basic and acidic residues | ||||
Sequence: KGGIGKKGESEENGECGL | ||||||
Region | 354-373 | Disordered | ||||
Sequence: KGGIGKKGESEENGECGLNR | ||||||
Region | 381-462 | Disordered | ||||
Sequence: TVGICTKGGDQRRESKTNDQGEGEYAEEEGDHCESEDSGKDAAENAEENASEGSGKYADGCPSDHTDKESSDEMSECENSFT | ||||||
Compositional bias | 389-403 | Basic and acidic residues | ||||
Sequence: GDQRRESKTNDQGEG | ||||||
Compositional bias | 413-427 | Basic and acidic residues | ||||
Sequence: CESEDSGKDAAENAE | ||||||
Compositional bias | 441-455 | Basic and acidic residues | ||||
Sequence: CPSDHTDKESSDEMS |
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length607
- Mass (Da)69,542
- Last updated2016-10-05 v1
- ChecksumA245BBFB0D279B4F
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 354-371 | Basic and acidic residues | ||||
Sequence: KGGIGKKGESEENGECGL | ||||||
Compositional bias | 389-403 | Basic and acidic residues | ||||
Sequence: GDQRRESKTNDQGEG | ||||||
Compositional bias | 413-427 | Basic and acidic residues | ||||
Sequence: CESEDSGKDAAENAE | ||||||
Compositional bias | 441-455 | Basic and acidic residues | ||||
Sequence: CPSDHTDKESSDEMS |
Keywords
- Technical term