A0A182DXM0 · A0A182DXM0_ONCOC
- ProteinMultifunctional fusion protein
- GeneKATNA1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1518 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation.
Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.
Catalytic activity
Cofactor
Note: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.
Activity regulation
ATPase activity is stimulated by microtubules, which promote homooligomerization. ATP-dependent microtubule severing is stimulated by interaction with KATNB1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 375-382 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GPPGTGKT | ||||||
Binding site | 824 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 838 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 841 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 847 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 878-885 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GKGGVGKS | ||||||
Binding site | 1052 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); ligand shared with heterodimeric partner | ||||
Sequence: C | ||||||
Binding site | 1055 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); ligand shared with heterodimeric partner | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cell projection | |
Cellular Component | centrosome | |
Cellular Component | cytoplasm | |
Cellular Component | microtubule | |
Cellular Component | spindle pole | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | acyltransferase activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | ATP-dependent FeS chaperone activity | |
Molecular Function | isomerase activity | |
Molecular Function | metal ion binding | |
Molecular Function | microtubule binding | |
Molecular Function | microtubule severing ATPase activity | |
Biological Process | cell division | |
Biological Process | DNA repair | |
Biological Process | iron-sulfur cluster assembly | |
Biological Process | microtubule severing |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameMultifunctional fusion protein
Including 2 domains:
- Recommended nameKatanin p60 ATPase-containing subunit A1
- EC number
- Short namesKatanin p60 subunit A1
- Alternative names
- Recommended nameCytosolic Fe-S cluster assembly factor NUBP1 homolog
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Spirurina > Spiruromorpha > Filarioidea > Onchocercidae > Onchocerca
Accessions
- Primary accessionA0A182DXM0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Predominantly cytoplasmic. Also localized to the interphase centrosome and the mitotic spindle poles. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1.
Keywords
- Cellular component
Interaction
Subunit
Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules. Interacts with KATNB1, which may serve as a targeting subunit.
Heterotetramer of 2 NUBP1 and 2 NUBP2 chains.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 224-308 | Disordered | ||||
Sequence: PPLTKKTSAIPSPKSAAKKQTYSRVDNKTKKSSVGKSPCTSAGCRNITKRANSLGRASMSELSEVTPKEEGDNNSNEKGGNEKVF | ||||||
Compositional bias | 255-283 | Polar residues | ||||
Sequence: SSVGKSPCTSAGCRNITKRANSLGRASMS | ||||||
Compositional bias | 286-308 | Basic and acidic residues | ||||
Sequence: SEVTPKEEGDNNSNEKGGNEKVF | ||||||
Domain | 1128-1514 | Ketosynthase family 3 (KS3) | ||||
Sequence: MKRVVITGIGIVSPFGVGKRLLFDNLLANNVALQHDEKLKIIVGRVSKYGENGLDLTSWSSRELKRMSRGSVLAVVAAEEAVKDAGLKECHMEETGVNVGMGIADLELIYDVGKQIAEGKGRRVTPFFIPRILTNMPAGHVSIKFGMRGPQLSSCTACATGLHSVGDSATFIRMGRAKRMLAGATEACVNSIAVIGFSQMRALTMTCSRPFDKRRDGFVLSEGAAILVLEEMEEALKRKANIYAEVLGYGVAGDAYHLTVPSEDGIGAFLSMERCLTDSSINPEQVTYVNAHATSTVLGDRFESLAIARLFPGHIGHTLAAAGAIETAITAMCVKESKLVGNVGLEESDIKENLRFLKQSEGWNNERVALVNSFGFGGSHATLCLSA |
Sequence similarities
Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily.
Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.
Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,518
- Mass (Da)166,883
- Last updated2016-09-07 v1
- ChecksumFA5B97953B04122A
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 255-283 | Polar residues | ||||
Sequence: SSVGKSPCTSAGCRNITKRANSLGRASMS | ||||||
Compositional bias | 286-308 | Basic and acidic residues | ||||
Sequence: SEVTPKEEGDNNSNEKGGNEKVF |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
UYRW01000038 EMBL· GenBank· DDBJ | VDK62061.1 EMBL· GenBank· DDBJ | Genomic DNA |