A0A178Y3B9 · A0A178Y3B9_SINSA

  • Protein
    Multifunctional fusion protein
  • Gene
    cysC
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

APS kinase catalyzes the synthesis of activated sulfate.
Catalyzes the synthesis of activated sulfate.
Proposed to provide activated sulfate for transfer to Nod factor. ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity.
With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric-sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.
Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site31-38GTP (UniProtKB | ChEBI)
Binding site110-114GTP (UniProtKB | ChEBI)
Binding site165-168GTP (UniProtKB | ChEBI)
Binding site467-474ATP (UniProtKB | ChEBI)
Active site541Phosphoserine intermediate

GO annotations

AspectTerm
Molecular Functionadenylylsulfate kinase activity
Molecular FunctionATP binding
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionsulfate adenylyltransferase (ATP) activity
Biological Processhydrogen sulfide biosynthetic process
Biological Processsulfate assimilation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Multifunctional fusion protein

Including 2 domains:

  • Recommended name
    Sulfate adenylyltransferase subunit 1
  • EC number
  • Alternative names
    • ATP-sulfurylase large subunit
    • Sulfate adenylate transferase
      (SAT
      )
  • Recommended name
    Adenylyl-sulfate kinase
  • EC number
  • Alternative names
    • APS kinase
    • ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
    • Adenosine-5'-phosphosulfate kinase

Gene names

    • Name
      cysC
    • Synonyms
      cysN
    • ORF names
      ATB98_06580

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • LMG 7837
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Rhizobiaceae > Sinorhizobium/Ensifer group > Sinorhizobium

Accessions

  • Primary accession
    A0A178Y3B9

Proteomes

PTM/Processing

Keywords

Interaction

Subunit

Heterodimer composed of CysD, the smaller subunit, and CysN.
Sulfate-activating enzymes, NodP and NodQ, may be physically associated.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain22-236Tr-type G

Sequence similarities

Belongs to the APS kinase family.
In the C-terminal section; belongs to the APS kinase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    633
  • Mass (Da)
    69,784
  • Last updated
    2016-09-07 v1
  • Checksum
    49972D738E8108D8
MSYVQSIPPHDIETHLAEHDNKSILRFMTCGSVDDGKSTLIGRLLYDAKLIFEDQLANLGRVGAPGAANGKEIDLALLLDGLEAEREQGITIDVAYRYFATSKRKFIVADTPGHEEYTRNMVTGASTADLAIILIDSRQGILQQTRRHSYIASLLGIRHVVLAVNKIDLVDFREQVFDEIVADYAVFAKELGFASVQPIPISARYGDNVISASANTPWYKGPALLEYLETVELDPTDGEKPFRFPVQLVMRPNADFRGYAGQVASGSVSVGDPVVVAKSGQRSSVKAIVTYDGNLETAAEGEAVTIVLADEVDASRGNMLVTPAARPFVADQFQAHVIWFDASPMLPGRSYILRTETDSVSATVTALKHQVNINSFLREAAKSLQMNEVGVCNFSTQTPIAFDAYKDNRATGNFIIVDRVTNATVGAGMIDFPLRRADNVHWHALEVNKGARSAMKHQRPAVLWFTGLSGSGKSTIANALDRILHARGKHTYLLDGDNVRHGLNRDLGFTEEDRVENIRRVAEVAKLMADAGLIVLVSFISPFRDERRMARELMEEGEFIEIFVDTPLDECARRDPKGLYEKALAGKIANFTGVSSPYEAPEKPELHLETVGYEPIELALRIEQFLDGRMDEK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LNQB01000083
EMBL· GenBank· DDBJ
OAP42068.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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