A0A178W755 · A0A178W755_ARATH
- ProteinThreonylcarbamoyladenosine tRNA methylthiotransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids601 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t6A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) at position 37 in tRNAs that read codons beginning with adenine.
Catalytic activity
- [sulfur carrier]-SH + AH2 + N6-L-threonylcarbamoyladenosine37 in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N6-L-threonylcarbamoyladenosine37 in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H+ + L-methionine + S-adenosyl-L-homocysteine
Cofactor
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | N6-threonylcarbomyladenosine methylthiotransferase activity | |
Molecular Function | tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase |
Keywords
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThreonylcarbamoyladenosine tRNA methylthiotransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionA0A178W755
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 580-600 | Helical | ||||
Sequence: VFVSSVTILVLLISIACRVLL |
Keywords
- Cellular component
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 30-53 | Polar residues | ||||
Sequence: PKTNKSKRISSKPDQITASNRDSL | ||||||
Region | 30-55 | Disordered | ||||
Sequence: PKTNKSKRISSKPDQITASNRDSLAP | ||||||
Domain | 64-172 | MTTase N-terminal | ||||
Sequence: QTIYIKTFGCSHNQSDSEYMAGQLSAFGYALTEVPEEADLWLINTCTVKSPSQSAMSTLITRGRSGKKPLVIAGCVPQGSRDLKELEGVSVVGVQQIDRVVEIVEETLK | ||||||
Domain | 193-425 | Radical SAM core | ||||
Sequence: RRNNFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVERVRTVISEGVKEIWLSSEDTGAYGRDIGVNLPILLNAIVKELPSDQSTMLRIGMTNPPFILEHLKEIAAVLRHPCVYTFLHVPVQSGSDSVLTAMNREYTASEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFSQTVELIKDYKFPQVHISQFYPRPGTPAAKMKKVQSKIVKQRSRELTSVFEAFAPY | ||||||
Domain | 423-488 | TRAM | ||||
Sequence: APYTGMECREERIWITEVATDGIHLVGHTKGYVQVLVTGPESMLGTSAMARITSVGRWSVFGEVIE |
Sequence similarities
Belongs to the methylthiotransferase family. CDKAL1 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length601
- Mass (Da)65,496
- Last updated2017-04-12 v1
- ChecksumAAC13F7EFCE3DA3D
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 30-53 | Polar residues | ||||
Sequence: PKTNKSKRISSKPDQITASNRDSL |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LUHQ01000001 EMBL· GenBank· DDBJ | OAP13551.1 EMBL· GenBank· DDBJ | Genomic DNA |