A0A178VHJ5 · A0A178VHJ5_ARATH
- ProteinKetol-acid reductoisomerase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids591 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
Catalytic activity
- (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-acetolactate + H+ + NADPH
Cofactor
Note: Binds 2 magnesium ions per subunit.
Pathway
Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.
Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 220 | |||||
Sequence: H | ||||||
Binding site | 309 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 309 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 313 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 371 | substrate | ||||
Sequence: S | ||||||
Binding site | 486 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 490 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 512 | substrate | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ketol-acid reductoisomerase activity | |
Molecular Function | metal ion binding | |
Biological Process | isoleucine biosynthetic process | |
Biological Process | valine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameKetol-acid reductoisomerase
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionA0A178VHJ5
- Secondary accessions
Proteomes
Genome annotation databases
Expression
Gene expression databases
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-20 | Disordered | ||||
Sequence: MAAATSSIAPSLSCPSPSSS | ||||||
Domain | 102-300 | KARI N-terminal Rossmann | ||||
Sequence: VRGGRDLFKHLPDAFKGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKIGLRKGSRSFEEARAAGFTEESGTLGDIWETIAGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLDFPKNISVVAVCPKGMGPSVRRLYVQGKEINGAGINASFAVHQDVDGRAADVALGWSVALGSPFTFAT | ||||||
Domain | 301-449 | KARI C-terminal knotted | ||||
Sequence: TLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVECITGTISRTISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGSEIRSVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAG | ||||||
Domain | 450-586 | KARI C-terminal knotted | ||||
Sequence: DLGPLYPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPEL |
Sequence similarities
Belongs to the ketol-acid reductoisomerase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length591
- Mass (Da)63,812
- Last updated2016-09-07 v1
- Checksum003C41A69B2C0F4F
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CACSHJ010000089 EMBL· GenBank· DDBJ | CAA0387255.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR881468 EMBL· GenBank· DDBJ | CAD5326197.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LUHQ01000003 EMBL· GenBank· DDBJ | OAP05158.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CACRSJ010000106 EMBL· GenBank· DDBJ | VYS60806.1 EMBL· GenBank· DDBJ | Genomic DNA |